INSERM, U922 - LHEA, Faculté de Médecine, rue haute de reculée, 49045 Angers Cedex, France.
Micron. 2010 Jun;41(4):321-6. doi: 10.1016/j.micron.2009.12.005. Epub 2009 Dec 22.
Sinus lift elevation is an interesting method to restore bone mass at the maxilla in edentulated patients. We have investigated the histological effects of beta tricalcium phosphate (beta-TCP) combined with autograft bone for sinus lift elevation. A series of 14 patients who were candidate for dental implantation were grafted with beta-TCP granules and morcellized autograft bone harvested at the chin. beta-TCP was characterized by scanning electron microscopy and optical profilometry. Before implant placement, a small bone biopsy (2mm in diameter) was done. The amount of residual material and newly formed bone were determined by microcomputed tomography. Histological analysis was done on undecalcified sections stained by Goldner's trichrome and osteoclast identification (TRAcP). beta-TCP served as a template for bone apposition by osteoblasts onto the granules' surface. The material was simultaneously resorbed by TRAcP positive osteoclasts and macrophages. Fragments of the material remained buried in bone trabeculae as long as 12 months post-graft but the formed bone onto the granules surface had a lamellar texture. beta-TCP combined with autograft bone appears a suitable biomaterial for sinus lift augmentation before the placement of bone implants. The material favors the apposition of lamellar bone by osteoblasts and is simultaneous resorbed by two types of cells.
窦提升是一种在无牙患者中恢复上颌骨骨量的有趣方法。我们研究了β-磷酸三钙（β-TCP）与自体骨联合用于窦提升的组织学效果。一系列 14 名适合牙种植的患者接受了β-TCP 颗粒和颏部采集的碎化自体骨移植。β-TCP 通过扫描电子显微镜和光学轮廓仪进行了表征。在植入物放置之前，进行了小的骨活检（直径 2mm）。通过微计算机断层扫描确定残留材料和新形成骨的量。对 Goldner 三色染色和破骨细胞鉴定（TRAcP）的未脱钙切片进行组织学分析。β-TCP 作为成骨细胞在颗粒表面附着的模板。材料同时被 TRAcP 阳性破骨细胞和巨噬细胞吸收。在移植后 12 个月，材料的碎片仍然埋在骨小梁中，但在颗粒表面形成的骨具有板层结构。β-TCP 与自体骨联合使用似乎是在植入骨植入物之前进行窦提升增强的合适生物材料。该材料有利于成骨细胞附着板层骨，并同时被两种类型的细胞吸收。
