Laboratory of Microbiology, Xiamen Inspection & Quarantine Technology Center, Xiamen, 361026, PR China.
Shanghai Key Laboratory of Bio-energy Crops, School of Life Sciences, Shanghai University, Shanghai, 200444, PR China.
Int J Syst Evol Microbiol. 2010 Dec;60(Pt 12):2735-2738. doi: 10.1099/ijs.0.017590-0. Epub 2010 Jan 8.
A spore-forming anaerobic bacterium, designated strain P6(T), was isolated from the sludge of an up-flow anaerobic sludge blanket reactor treating brewery wastewater. Cells were Gram-positive, oval and 0.6-0.9 μm by 1.2-1.8 μm in size. Growth was observed at 20-42 °C and at pH 5.0-7.5. It fermented several hexoses, polysaccharides and alcohols. Sucrose and aesculin could also be fermented. The main end products of fermentation from glucose were acetate, lactate and fumarate; trace CO(2) and H(2) were also produced. The DNA G+C content of strain P6(T) was 55.6 mol%. The major cellular fatty acids were iso-C(15 : 0), anteiso-C(15 : 0) and iso-C(14 : 0) 3-OH. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain P6(T) represented a novel phyletic sublineage in clostridial cluster III, and showed <91 % similarity to the type strains of recognized species in this cluster. Phenotypically, the new isolate was distinguished from its phylogenetic relatives (e.g. Clostridium straminisolvens, Clostridium thermocellum, Acetivibrio cellulolyticus and Clostridium aldrichii) by producing acid from glucose and its inability to degrade cellulose. On the basis of evidence from this polyphasic study, strain P6(T) is considered to represent a novel species of a new genus, for which the name Saccharofermentans acetigenes gen. nov., sp. nov. is proposed. The type strain of Saccharofermentans acetigenes is P6(T) (=JCM 14006(T) =AS 1.5064(T)).
一株产孢子的厌氧细菌，被命名为 P6(T) 菌株，是从处理啤酒废水的上流式厌氧污泥床反应器的污泥中分离出来的。细胞呈革兰氏阳性，椭圆形，大小为 0.6-0.9μm×1.2-1.8μm。在 20-42°C 和 pH5.0-7.5 的条件下可以生长。它可以发酵几种己糖、多糖和醇。蔗糖和马栗树皮苷也可以发酵。从葡萄糖发酵的主要末端产物是乙酸盐、乳酸盐和富马酸盐；还产生痕量的 CO(2)和 H(2)。P6(T) 菌株的 DNA G+C 含量为 55.6mol%。主要的细胞脂肪酸是 iso-C(15 : 0)、anteiso-C(15 : 0)和 iso-C(14 : 0) 3-OH。基于 16S rRNA 基因序列的系统发育分析表明，P6(T) 菌株代表梭菌 III 簇中的一个新的分支，与该簇中公认的种的模式株的相似性<91%。表型上，新分离株与亲缘关系较近的菌株（如 Clostridium straminisolvens、Clostridium thermocellum、Acetivibrio cellulolyticus 和 Clostridium aldrichii）不同，它可以从葡萄糖中产生酸，并且不能降解纤维素。基于多相研究的证据，P6(T) 菌株被认为代表了一个新属的新种，建议将其命名为 Saccharofermentans acetigenes gen. nov.，sp. nov.。Saccharofermentans acetigenes 的模式菌株是 P6(T)（=JCM 14006(T)=AS 1.5064(T)）。
