gen. nov., sp. nov., and sp. nov., gen. nov.: novel bacteria from the family isolated from the female genital tract.

Four obligately anaerobic Gram-positive bacteria representing one novel genus and two novel species were isolated from the female genital tract. Both novel species, designated UPII 610-J and KA00274, and an additional isolate of each species were characterized utilizing biochemical, genotypic and phylogenetic analyses. All strains were non-motile and non-spore forming, asaccharolytic, non-cellulolytic and indole-negative coccobacilli. Fatty acid methyl ester analysis for UPII 610-J and KA00274 and additional isolates revealed C, C, Cω9c and Cω6,9c to be the major fatty acids for both species. UPII 610-J had a 16S rRNA gene sequence similarity of 99.4 % to an uncultured clone sequence (AY724740) designated as Bacterial Vaginosis Associated Bacterium 2 (BVAB2). KA00274 had a 16S rRNA gene sequence similarity of 96.5 % to UPII 610-J. Whole genomic DNA mol% G+C content was 42.2 and 39.3 % for UPII 610-JT and KA00274, respectively. Phylogenetic analyses indicate these isolates represent a novel genus and two novel species within the family. We propose the names gen. nov., sp. nov., for UPII 610-J representing the type strain of this species (=DSM 112989, =ATCC TSD-274) and gen. nov., sp. nov., for KA00274 representing the type strain of this species (=DSM 112988, =ATCC TSD-275).