Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA, USA.
Magee-Womens Research Institute, Pittsburgh, PA, USA.
Int J Syst Evol Microbiol. 2023 Oct;73(10). doi: 10.1099/ijsem.0.006017.
Four obligately anaerobic Gram-positive bacteria representing one novel genus and two novel species were isolated from the female genital tract. Both novel species, designated UPII 610-J and KA00274, and an additional isolate of each species were characterized utilizing biochemical, genotypic and phylogenetic analyses. All strains were non-motile and non-spore forming, asaccharolytic, non-cellulolytic and indole-negative coccobacilli. Fatty acid methyl ester analysis for UPII 610-J and KA00274 and additional isolates revealed C, C, Cω9c and Cω6,9c to be the major fatty acids for both species. UPII 610-J had a 16S rRNA gene sequence similarity of 99.4 % to an uncultured clone sequence (AY724740) designated as Bacterial Vaginosis Associated Bacterium 2 (BVAB2). KA00274 had a 16S rRNA gene sequence similarity of 96.5 % to UPII 610-J. Whole genomic DNA mol% G+C content was 42.2 and 39.3 % for UPII 610-JT and KA00274, respectively. Phylogenetic analyses indicate these isolates represent a novel genus and two novel species within the family. We propose the names gen. nov., sp. nov., for UPII 610-J representing the type strain of this species (=DSM 112989, =ATCC TSD-274) and gen. nov., sp. nov., for KA00274 representing the type strain of this species (=DSM 112988, =ATCC TSD-275).
从女性生殖道中分离到 4 株严格厌氧革兰氏阳性细菌，代表一个新属和两个新种。两种新种，分别命名为 UPII 610-J 和 KA00274，以及每种的另一个分离株，通过生化、基因型和系统发育分析进行了特征描述。所有菌株均为非运动性、非孢子形成的、无发酵性、非纤维素分解性和吲哚阴性的球杆菌。UPII 610-J 和 KA00274 以及其他分离株的脂肪酸甲酯分析表明，两种细菌的主要脂肪酸均为 C、C、Cω9c 和 Cω6,9c。UPII 610-J 的 16S rRNA 基因序列与未培养的克隆序列（AY724740）相似度为 99.4%，被命名为细菌性阴道病相关细菌 2（BVAB2）。KA00274 与 UPII 610-J 的 16S rRNA 基因序列相似度为 96.5%。全基因组 DNA mol% G+C 含量分别为 UPII 610-JT 和 KA00274 的 42.2%和 39.3%。系统发育分析表明，这些分离株代表了一个新属和该科中的两个新种。我们提议 UPII 610-J 为代表该种的新种（=DSM 112989，=ATCC TSD-274）和 KA00274 为代表该种的新种（=DSM 112988，=ATCC TSD-275）。
