Division of Dermatology, CHU Sainte-Justine, University of Montreal, Montreal, QC, Canada.
Mod Pathol. 2010 Mar;23(3):376-80. doi: 10.1038/modpathol.2009.163. Epub 2010 Jan 8.
Cancer stem cells expressing CD133 exist in a wide array of tumors and their identification in malignant melanoma may help refine classification, diagnosis and treatment. To study the correlation between CD133 expression in childhood melanoma and lymph node and/or visceral metastasis, we evaluated 12 cases of malignant melanoma and 12 control cases of Spitz nevus occurring in children. Double immunostaining with CD133 and Ki-67 was performed in the cases showing CD133 positivity. Three melanoma patients had lymph node metastasis and only one had multivisceral metastases; CD133 was positive only in these four patients. The Ki-67 index was lower in the CD133(+) cells in comparison with the CD133(-) melanoma cells in three cases. We found no positivity for CD133 in all the Spitz nevi. CD133(+) cancer stem cell expression in childhood malignant melanoma might correlate with lymph node and/or visceral metastasis and may have a low proliferative Ki-67 index that might explain their chemoresistance.
肿瘤中存在表达 CD133 的癌症干细胞，其在恶性黑色素瘤中的鉴定可能有助于完善分类、诊断和治疗。为了研究儿童黑色素瘤中 CD133 表达与淋巴结和/或内脏转移之间的相关性，我们评估了 12 例恶性黑色素瘤和 12 例儿童 Spitz 痣对照病例。在显示 CD133 阳性的病例中进行了 CD133 和 Ki-67 的双重免疫染色。3 例黑色素瘤患者发生淋巴结转移，仅 1 例发生多脏器转移；仅这 4 例患者的 CD133 阳性。与 CD133(-)黑色素瘤细胞相比，在 3 例患者中 CD133(+)细胞的 Ki-67 指数较低。我们在所有 Spitz 痣中均未发现 CD133 阳性。儿童恶性黑色素瘤中 CD133(+)癌症干细胞的表达可能与淋巴结和/或内脏转移相关，并且可能具有较低的增殖 Ki-67 指数，这可能解释了它们的化疗耐药性。
