CD133和KAI1/CD82表达标记的癌干细胞在喉鳞状细胞癌中的临床病理意义
Clinicopathological significance of cancer stem cells marked by CD133 and KAI1/CD82 expression in laryngeal squamous cell carcinoma.
作者信息
Yu Lan, Zhou Lei, Wu Shiwu, Gong Xiaomeng, Feng Zhenzhong, Ma Li, Zhu Bo, Yao Nan, Wang Danna, Dong Huiming
机构信息
Department of Pathology, the First Hospital Affiliated to Bengbu Medical College, Bengbu Medical College, 800 Zhihuai Ave, Longzihu, Bengbu, Anhui, China.
出版信息
World J Surg Oncol. 2014 Apr 24;12:118. doi: 10.1186/1477-7819-12-118.
BACKGROUND
Presently, CD133 is one of the hottest markers to characterize cancer stem cells and KAI1/CD82 is reported as an important marker for the metastasis and prognosis of many cancers. The purpose of our study is to explore the relationship between cancer stem cells (CSCs) marked by CD133 and KAI1/CD82 expression and the clinicopathological characteristics of patients with laryngeal squamous cell carcinoma (LSCC).
METHODS
Immunohistochemical analysis was used to detect the expression of CD133 and KAI1/CD82 in 83 archival surgical specimens of human LSCC and 83 cases of normal laryngeal tissues.
RESULTS
In LSCC, positive rates of 49.4% and 41.0% were obtained for CD133 and KAI1/CD82, respectively. The expression of CD133 in LSCC tissues was significantly higher than that in normal tissues (P<0.001), and the expression of CD133 was positively associated with pTNM stage (P=0.005), pathological grade (P=0.001), and lymph node metastasis (P<0.001). The reduced expression of KAI1/CD82 was present in LSCC tissues. The positive rate of KAI1/CD82 expression was negatively correlated with pTNM stage (P=0.014), pathological grade (P<0.001), and lymph node metastasis (P=0.007). A correlation analysis showed that there was a negative relationship between the expression of CD133 and KAI1/CD82 protein in LSCC tissues (P<0.001). By Kaplan-Meier analysis, the expression of CD133 was negatively correlated with overall survival (OS) (log-rank=40.949, P<0.001) and disease-free survival (DFS) (log-rank=39.307, P<0.001) time of LSCC. The expression of KAI1/CD82 was positively correlated with OS (log-rank=40.279, P<0.001) and DFS (log-rank=39.271, P<0.001) time of LSCC. Cox regression analysis: the expression of CD133 and KAI1/CD82, and pTNM stages were independent prognostic factors of LSCC (P<0.05).
CONCLUSIONS
Thus the detection of CD133 and KAI1/CD82 proteins may be used as a potential indicator of LSCC prognosis.
背景
目前，CD133是鉴定癌症干细胞的热门标志物之一，而KAI1/CD82被报道为多种癌症转移和预后的重要标志物。本研究的目的是探讨以CD133标记的癌症干细胞与KAI1/CD82表达之间的关系，以及喉鳞状细胞癌（LSCC）患者的临床病理特征。
方法
采用免疫组织化学分析法检测83例人LSCC手术存档标本及83例正常喉组织中CD133和KAI1/CD82的表达。
结果
在LSCC中，CD133和KAI1/CD82的阳性率分别为49.4%和41.0%。LSCC组织中CD133的表达显著高于正常组织（P<0.001），且CD133的表达与pTNM分期（P=0.005）、病理分级（P=0.001）及淋巴结转移（P<0.001）呈正相关。LSCC组织中存在KAI1/CD82表达降低的情况。KAI1/CD82表达的阳性率与pTNM分期（P=0.014）、病理分级（P<0.001）及淋巴结转移（P=0.007）呈负相关。相关性分析显示，LSCC组织中CD133与KAI1/CD82蛋白的表达呈负相关（P<0.001）。通过Kaplan-Meier分析，CD133的表达与LSCC的总生存期（OS）（对数秩检验=40.949，P<0.001）和无病生存期（DFS）（对数秩检验=39.307，P<0.001）时间呈负相关。KAI1/CD82的表达与LSCC的OS（对数秩检验=40.279，P<0.001）和DFS（对数秩检验=39.271，P<0.001）时间呈正相关。Cox回归分析：CD133和KAI1/CD82的表达以及pTNM分期是LSCC的独立预后因素（P<0.05）。
结论
因此，检测CD133和KAI1/CD82蛋白可能作为LSCC预后的潜在指标。
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