LEM-EA3514-Universite Paris Diderot-Paris 7, 2 place Jussieu, 75251 Paris cedex 05, France.
Mol Plant Microbe Interact. 2010 Feb;23(2):139-43. doi: 10.1094/MPMI-23-2-0139.
Pectobacterium carotovorum are economically important plant pathogens that cause plant soft rot. These enterobacteria display high diversity world-wide. Their pathogenesis depends on production and secretion of virulence factors such as plant cell wall-degrading enzymes, type III effectors, a necrosis-inducing protein, and a secreted virulence factor from Xanthomonas spp., which are tightly regulated by quorum sensing. Pectobacterium carotovorum also present pathogen-associated molecular patterns that could participate in their pathogenicity. In this study, by using suspension cells of Arabidopsis thaliana, we correlate plant cell death and pectate lyase activities during coinfection with different P. carotovorum strains. When comparing soft rot symptoms induced on potato slices with pectate lyase activities and plant cell death observed during coculture with Arabidopsis thaliana cells, the order of strain virulence was found to be the same. Therefore, Arabidopsis thaliana cells could be an alternative tool to evaluate rapidly and efficiently the virulence of different P. carotovorum strains.
果胶杆菌是一类重要的经济植物病原菌，可引起植物软腐病。这些肠杆菌在全球范围内具有高度多样性。它们的致病性依赖于产生和分泌毒力因子，如植物细胞壁降解酶、III 型效应子、一种坏死诱导蛋白和一种来自黄单胞菌属的分泌毒力因子，这些因子受到群体感应的严格调控。果胶杆菌还存在与致病性相关的病原体相关分子模式。在这项研究中，我们通过使用拟南芥悬浮细胞，在与不同果胶杆菌菌株共同感染时，将植物细胞死亡与果胶裂解酶活性相关联。在比较马铃薯切片上诱导的软腐病症状与与拟南芥细胞共培养时观察到的果胶裂解酶活性和植物细胞死亡时，发现菌株毒力的顺序是相同的。因此，拟南芥细胞可以成为一种快速、有效地评估不同果胶杆菌菌株毒力的替代工具。
