California Institute for Medical Research, 2260 Clove Dr, San Jose, CA 95128, United States.
Immunol Lett. 2010 Feb 16;128(2):105-7. doi: 10.1016/j.imlet.2009.12.021. Epub 2010 Jan 12.
Mannose binding lectin (MBL) is a protein of the collectin family that appears important in resistance to invasive pulmonary aspergillosis. We assessed the role of MBL in experimental systemic aspergillosis. MBL-sufficient C57BL/6 (WT) mice and B6.129S4--Mb11(tm1Kata) Mb12(tm1Kata)/J MBL A and C gene-knockout (KO) mice were infected intravenously with different inocula of Aspergillus fumigatus conidia. WT and KO mice were dose-responsively susceptible. In no instance were the KO mice more susceptible than WT. At the highest inoculum, all WT and 90% of KO mice died on day 4 (P>0.05). Reduction of the inoculum to 5.5 x 10(6) conidia was lethal, but comparison showed KO mice less susceptible to lethal infection (P<0.015). At the lowest inoculum used, deaths of KO mice were delayed, but survival was not significantly different than WT (P>0.05). These results suggest MBL may play a deleterious role in systemic aspergillosis.
甘露聚糖结合凝集素（MBL）是一种属于凝集素家族的蛋白质，在抵抗侵袭性肺曲霉病方面似乎很重要。我们评估了 MBL 在实验性系统性曲霉病中的作用。用不同剂量的烟曲霉分生孢子静脉感染 MBL 充足的 C57BL/6（WT）小鼠和 B6.129S4--Mb11(tm1Kata) Mb12(tm1Kata)/J MBL A 和 C 基因敲除（KO）小鼠。WT 和 KO 小鼠均对剂量呈敏感性。在任何情况下，KO 小鼠均不比 WT 小鼠更易感。在最高接种量下，所有 WT 和 90%的 KO 小鼠均于第 4 天死亡（P>0.05）。将接种量减少到 5.5 x 10(6)个分生孢子可致死，但比较表明 KO 小鼠对致死性感染的敏感性降低（P<0.015）。在使用的最低接种量下，KO 小鼠的死亡被延迟，但与 WT 相比，存活时间无显著差异（P>0.05）。这些结果表明 MBL 可能在系统性曲霉病中发挥有害作用。
