Terashima Angélica, Marcos Luis, Maco Vicente, Canales Marco, Samalvides Frine, Tello Raúl
Instituto de Medicina Tropical Alexander Von Humboldt (IMTAVH), Universidad Peruana Cayetano Heredia, Lima, Perú.
Rev Gastroenterol Peru. 2009 Oct-Dec;29(4):305-10.
The objective of this study was to evaluate the diagnostic efficacy of the Spontaneous Sedimentation in Tube Technique (SSTT) described by Tello, in comparison with the direct smear and other concentration techniques, when it is used to determine the prevalence of intestinal parasites in field and laboratory studies in rural areas of the Andean Region.
A prospective study (2000-2004) was carried out including 1 802 fecal samples from several areas around Peru: Iquitos (N = 74), Puno (N = 399), Junín (N = 1241), Lima (N = 88).
SSTT showed more sensitivity to detect helminthes and protozoa in comparison to other conventional techniques carried out simultaneously (P;0.000). Although Baermann Technique and Lumbrera's Rapid Sedimentation Technique are crucial for Strongyloides larvae and Fasciola eggs detection, respectively; SSTT contributes to an effective and timely diagnosis of the intestinal parasites.
Due to its low cost, easy reproducibility and adaptability both in the laboratory and field work, it should be mandatory and urgent its implementation in laboratories from rural areas, as well as training of the staff involved in the diagnosis, as first step in the fight against intestinal parasites in Peru.
本研究的目的是评估特略所描述的试管内自然沉淀技术(SSTT)在安第斯地区农村地区的现场和实验室研究中用于确定肠道寄生虫患病率时,与直接涂片法和其他浓缩技术相比的诊断效力。
开展了一项前瞻性研究(2000 - 2004年),纳入了来自秘鲁多个地区的1802份粪便样本:伊基托斯(N = 74)、普诺(N = 399)、胡宁(N = 1241)、利马(N = 88)。
与同时进行的其他传统技术相比,SSTT在检测蠕虫和原生动物方面表现出更高的灵敏度(P < 0.000)。虽然贝尔曼技术和伦布雷拉快速沉淀技术分别对粪类圆线虫幼虫和肝片吸虫卵的检测至关重要,但SSTT有助于对肠道寄生虫进行有效且及时的诊断。
由于其成本低、易于重复且在实验室和现场工作中都具有适应性,应在秘鲁农村地区的实验室强制且尽快实施该技术,并对参与诊断的工作人员进行培训,这是秘鲁抗击肠道寄生虫的第一步。