College of Pharmacy and Research institute of Pharmaceutical Science, Seoul National University, Seoul 151-742, Korea.
Phytochem Anal. 2010 Jul-Aug;21(4):314-21. doi: 10.1002/pca.1201.
Pulsatilla koreana Nakai, with triterpenoidal saponins as its main pharmacological effective compounds, is known to have several biological activities, including hypoglycaemic, antitumour, cognition-enhancing, neuroprotective, cytotoxic and antiangiogenic activities. However, few analytical methods have been reported on the quality assessment of P. koreana roots.
To establish a high-performance liquid chromatography coupled with evaporative light scattering detection for the simultaneous determination of five triterpenoidal saponins, including pulsatilloside E (1), pulsatilla saponin H (2), anemoside B4 (3), hederacolchiside E (4) and cussosaponin C (5) in P. koreana.
The chromatographic separation was performed on a Shiseido CapCell PAK C18 analytical column efficiently using gradient elution with acetonitrile and water.
All calibration curves showed excellent linear regressions (R(2) > 0.9996) within the range of tested concentrations. The intra- and inter-day variations were below 4.78% in terms of RSD. The recoveries were 94.82-102.97% with RSD of 0.27-3.92% for spiked P. koreana samples.
The validated method was successfully used for the analysis of five saponins in P. koreana from different locations. Moreover, the different samples were clustered in accordance with contents of triterpenoidal saponins based on aglycon type by a principal component analysis.
白头翁（Pulsatilla koreana Nakai）以其主要的药理活性化合物三萜皂苷而闻名，具有多种生物活性，包括降血糖、抗肿瘤、增强认知、神经保护、细胞毒性和抗血管生成活性。然而，关于白头翁根的质量评估，很少有分析方法被报道。
建立一种高效液相色谱-蒸发光散射检测法，用于同时测定白头翁中的 5 种三萜皂苷，包括白头翁皂苷 E（1）、白头翁皂苷 H（2）、芹糖苷 B4（3）、hederacolchiside E（4）和 cussosaponin C（5）。
采用 Shiseido CapCell PAK C18 分析柱，以乙腈和水为流动相进行梯度洗脱，对色谱分离条件进行了优化。
所有校准曲线在测试浓度范围内均呈现出极好的线性关系（R²>0.9996）。日内和日间变异度以 RSD 计低于 4.78%。加标白头翁样品的回收率为 94.82%-102.97%，RSD 为 0.27%-3.92%。
该方法已成功用于不同产地白头翁中 5 种皂苷的分析。此外，根据主成分分析，基于苷元类型，不同样品根据三萜皂苷含量聚类。
