植物皂苷的高效液相色谱分析：2005 - 2010年最新进展
High-performance liquid chromatography analysis of plant saponins: An update 2005-2010.
作者信息
Negi Jagmohan S, Singh Pramod, Pant Geeta Joshi Nee, Rawat M S M
机构信息
Department of Chemistry, HNB Garhwal University, Srinagar (Garhwal).
出版信息
Pharmacogn Rev. 2011 Jul;5(10):155-8. doi: 10.4103/0973-7847.91109.
Abstract
Saponins are widely distributed in plant kingdom. In view of their wide range of biological activities and occurrence as complex mixtures, saponins have been purified and separated by high-performance liquid chromatography using reverse-phase columns at lower wavelength. Mostly, saponins are not detected by ultraviolet detector due to lack of chromophores. Electrospray ionization mass spectrometry, diode array detector , evaporative light scattering detection, and charged aerosols have been used for overcoming the detection problem of saponins.
摘要
皂苷广泛分布于植物界。鉴于其广泛的生物活性以及以复杂混合物形式存在，皂苷已通过使用反相柱在较低波长下的高效液相色谱法进行纯化和分离。大多数情况下，由于缺乏发色团，紫外检测器无法检测到皂苷。电喷雾电离质谱、二极管阵列检测器、蒸发光散射检测和带电气溶胶已被用于克服皂苷的检测问题。
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