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J Liq Chromatogr Relat Technol. 2010 Jan 1;33(4):563-571. doi: 10.1080/10826070903574659.
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Comparison between evaporative light scattering detection and charged aerosol detection for the analysis of saikosaponins.比较蒸发光散射检测和带电气溶胶检测在分析柴胡皂苷中的应用。
J Chromatogr A. 2010 Jun 25;1217(26):4347-54. doi: 10.1016/j.chroma.2010.04.047. Epub 2010 Apr 22.
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Simultaneous analysis of isoflavones and saponins in Pueraria flowers using HPLC coupled to an evaporative light scattering detector and isolation of a new isoflavone diglucoside.采用高效液相色谱-蒸发光散射检测法同时分析葛花中的异黄酮和皂苷，并分离得到一种新的异黄酮双糖苷。
J Nat Med. 2010 Jul;64(3):313-20. doi: 10.1007/s11418-010-0411-z. Epub 2010 Mar 31.
4
Determination of steroidal glycosides in Yucca gloriosa flowers by LC/MS/MS.采用 LC/MS/MS 法测定丝兰花朵中的甾体糖苷。
J Pharm Biomed Anal. 2010 Sep 5;52(5):791-5. doi: 10.1016/j.jpba.2010.02.033. Epub 2010 Mar 3.
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Simultaneous determination of three major bioactive saponins of Panax notoginseng using liquid chromatography-tandem mass spectrometry and a pharmacokinetic study.采用液相色谱-串联质谱法同时测定三七中三种主要生物活性皂苷及其药代动力学研究。
Chin Med. 2010 Mar 23;5:12. doi: 10.1186/1749-8546-5-12.
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Quality control of Pulsatilla koreana based on the simultaneous determination of triterpenoidal saponins by HPLC-ELSD and principal component analysis.基于 HPLC-ELSD 和主成分分析同时测定白头翁中三萜皂苷的质量控制。
Phytochem Anal. 2010 Jul-Aug;21(4):314-21. doi: 10.1002/pca.1201.
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Qualitative and quantitative analysis of steroidal saponins in crude extracts from Paris polyphylla var. yunnanensis and P. polyphylla var. chinensis by high performance liquid chromatography coupled with mass spectrometry.采用高效液相色谱-质谱联用法对滇重楼和七叶一枝花粗提物中的甾体皂苷进行定性和定量分析。
J Pharm Biomed Anal. 2010 Jan 5;51(1):114-24. doi: 10.1016/j.jpba.2009.08.020. Epub 2009 Aug 25.
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[Determination of two saponins in Tupistra chinensis rhizomes by RP-HPLC].反相高效液相色谱法测定开口箭根茎中的两种皂苷
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植物皂苷的高效液相色谱分析：2005 - 2010年最新进展

High-performance liquid chromatography analysis of plant saponins: An update 2005-2010.

作者信息

Negi Jagmohan S, Singh Pramod, Pant Geeta Joshi Nee, Rawat M S M

机构信息

Department of Chemistry, HNB Garhwal University, Srinagar (Garhwal).

出版信息

Pharmacogn Rev. 2011 Jul;5(10):155-8. doi: 10.4103/0973-7847.91109.

DOI:10.4103/0973-7847.91109
PMID:22303089
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3263049/
Abstract

Saponins are widely distributed in plant kingdom. In view of their wide range of biological activities and occurrence as complex mixtures, saponins have been purified and separated by high-performance liquid chromatography using reverse-phase columns at lower wavelength. Mostly, saponins are not detected by ultraviolet detector due to lack of chromophores. Electrospray ionization mass spectrometry, diode array detector , evaporative light scattering detection, and charged aerosols have been used for overcoming the detection problem of saponins.

摘要

皂苷广泛分布于植物界。鉴于其广泛的生物活性以及以复杂混合物形式存在，皂苷已通过使用反相柱在较低波长下的高效液相色谱法进行纯化和分离。大多数情况下，由于缺乏发色团，紫外检测器无法检测到皂苷。电喷雾电离质谱、二极管阵列检测器、蒸发光散射检测和带电气溶胶已被用于克服皂苷的检测问题。