College of Veterinary Medicine, Key Laboratory of Development and Evaluation of the Chemical and Herbal Drugs for Animal Use, China Agricultural University, 100193 Beijing, China.
Parasitol Res. 2010 Feb;106(3):607-13. doi: 10.1007/s00436-009-1704-6.
Ear mange mite Psoroptes cuniculi, one of the predominant parasites in rabbits, can cause considerable weight loss, low favorable fee conversion rates, and meningitis. The present experiment was to investigate the difference of plasma disposition and the variation of clinical efficacy under the effect of animal self-licking behavior in topically administered rabbits. Ten rabbits for pharmacokinetic study in two groups (the self-licking and the nonlicking)were topically administered with 1 mg kg(-1) of eprinomectin. In the self-licking group, rabbits were allowed to self-lick freely, while, to prevent self-licking, each animal in the non-licking group was fitted with a pet collar. Compared to the non-licking group, self-licking behavior contributed to an extremely significant shorter half-life of absorption (14.85+/-2.79 h in licking group vs.29.44+/-7.81 h in non-licking group, p<0.01) and an extremely significant higher C(max) value for eprinomectin (21.95+/-5.36 h in licking group vs. 6.98+/-0.72 ng ml(-1) in non-licking group, p<0.01) in plasma disposition. An extremely significantly shorter mean residence time (50.72+/-3.45 h) in self-licking group was also determined compared with the value obtained in non-licking group (106.66+/-7.39 h; p<0.01). Clinical efficacy study of eprinomectin was examined in rabbits naturally infested with P. cuniculi which were randomly allocated in three groups: the self-licking, the non-licking, and control groups. All rabbits in the self-licking and the non-licking groups were treated with topical eprinomectin at a single dose of 2 mg kg(-1) (day 0). Topical eprinomection led to a complete parasitological recovery in both treated groups on day 14 and remained free of live mites and clinical lesions from day 21 to the end of the study period (day 35).
耳螨寄生在兔子身上，是一种主要寄生虫，它会导致兔子体重明显下降、饲料转化率降低和脑膜炎。本实验旨在研究在动物自我舔舐行为的影响下，局部给予兔子时，血浆处置的差异和临床疗效的变化。在两组（舔舐组和非舔舐组）中，每组 10 只兔子进行药代动力学研究，以 1mgkg(-1)的依普菌素进行局部给药。在舔舐组中，允许兔子自由舔舐，而在非舔舐组中，为了防止兔子舔舐，每只动物都戴上了宠物项圈。与非舔舐组相比，舔舐行为导致吸收半衰期极显著缩短（舔舐组为 14.85+/-2.79h，非舔舐组为 29.44+/-7.81h，p<0.01），血浆中依普菌素的 C(max)值极显著升高（舔舐组为 21.95+/-5.36ngml(-1)，非舔舐组为 6.98+/-0.72ngml(-1)，p<0.01）。与非舔舐组相比，舔舐组的平均驻留时间也极显著缩短（50.72+/-3.45h）。在自然感染 P. cuniculi 的兔子中进行了依普菌素的临床疗效研究，将兔子随机分为三组：舔舐组、非舔舐组和对照组。舔舐组和非舔舐组的所有兔子均在第 0 天以 2mgkg(-1)的剂量局部给予依普菌素。局部给予依普菌素后，两组治疗组在第 14 天完全消除寄生虫，从第 21 天至研究结束（第 35 天）均无活螨和临床病变。
