Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Ministry of Education, Northeast Forestry University, Harbin, 150040, China.
Theor Appl Genet. 2010 May;120(7):1405-13. doi: 10.1007/s00122-010-1264-2. Epub 2010 Jan 13.
Vitamin E (VE) in soybean seed has value for foods, medicines, cosmetics, and animal husbandry. Selection for higher VE contents in seeds along with agronomic traits was an important goal for many soybean breeders. In order to map the loci controlling the VE content, F(5)-derived F(6) recombinant inbred lines (RILs) were advanced through single-seed-descent (SSD) to generate a population including 144 RILs. The population was derived from a cross between 'OAC Bayfield', a soybean cultivar with high VE content, and 'Hefeng 25', a soybean cultivar with low VE content. A total of 107 polymorphic simple sequence repeat markers were used to construct a genetic linkage map. Seed VE contents were analyzed by high performance liquid chromatography for multiple years and locations (Harbin in 2007 and 2008, Hulan in 2008 and Suihua in 2008). Four QTL associated with alpha-Toc (on four linkage groups, LGs), eight QTL associated with gamma-Toc (on eight LGs), four QTL associated with delta-Toc (on four LGs) and five QTL associated with total VE (on four LGs) were identified. A major QTL was detected by marker Satt376 on linkage group C2 and associated with alpha-Toc (0.0012 > P > 0.0001, 5.0% < R (2) < 17.0%, 25.1 < alpha-Toc < 30.1 microg g(-1)), total VE (P < 0.0001, 7.0% < R (2) < 10.0%, 118.2 < total VE < 478.3 microg g(-1)). A second QTL detected by marker Satt286 on LG C2 was associated with gamma-Toc (0.0003 > P > 0.0001, 6.0% < R (2) < 13.0%, 141.5 < gamma-Toc < 342.4 microg g(-1)) and total VE (P < 0.0001, 2.0% < R (2) < 9.0%, 353.9 < total VE < 404.0 microg g(-1)). Another major QTL was detected by marker Satt266 on LG D1b that was associated with alpha-Toc (0.0002 > P > 0.0001, 4.0% < R (2) < 6.0%, 27.7 < alpha-Toc < 43.7 microg g(-1)) and gamma-Toc (0.0032 > P > 0.0001, 3.0% < R (2) < 10.0%, 69.7 < gamma-Toc < 345.7 microg g(-1)). Since beneficial alleles were all from 'OAC Bayfield', it was concluded that these three QTL would have great potential value for marker assisted selection for high VE content.
维生素 E（VE）在大豆种子中具有食品、药品、化妆品和畜牧业的价值。选择具有更高种子 VE 含量和农艺性状的大豆品种是许多大豆育种者的重要目标。为了定位控制 VE 含量的基因座，通过单粒传代（SSD）将 F(5)衍生的 F(6)重组自交系（RIL）推进，生成包括 144 个 RIL 的群体。该群体来自高 VE 含量的大豆品种“OAC Bayfield”和低 VE 含量的大豆品种“Hefeng 25”之间的杂交。总共使用了 107 个多态性简单序列重复标记来构建遗传连锁图谱。通过高效液相色谱法对种子 VE 含量进行了多年和多个地点（2007 年和 2008 年在哈尔滨，2008 年在呼兰和绥化市）的分析。鉴定出与 alpha-Toc（四个连锁群，LG）相关的四个 QTL，与 gamma-Toc（八个 LG）相关的八个 QTL，与 delta-Toc（四个 LG）相关的四个 QTL 和与总 VE（四个 LG）相关的五个 QTL。在连锁群 C2 上的标记 Satt376 检测到一个主要 QTL，与 alpha-Toc（0.0012 > P > 0.0001，5.0% < R (2) < 17.0%，25.1 < alpha-Toc < 30.1 microg g(-1))，总 VE（P < 0.0001，7.0% < R (2) < 10.0%，118.2 < 总 VE < 478.3 microg g(-1))。在 LG C2 上的标记 Satt286 检测到的第二个 QTL 与 gamma-Toc（0.0003 > P > 0.0001，6.0% < R (2) < 13.0%，141.5 < gamma-Toc < 342.4 microg g(-1))和总 VE（P < 0.0001，2.0% < R (2) < 9.0%，353.9 < 总 VE < 404.0 microg g(-1))相关。在连锁群 D1b 上的标记 Satt266 检测到的另一个主要 QTL 与 alpha-Toc（0.0002 > P > 0.0001，4.0% < R (2) < 6.0%，27.7 < alpha-Toc < 43.7 microg g(-1))和 gamma-Toc（0.0032 > P > 0.0001，3.0% < R (2) < 10.0%，69.7 < gamma-Toc < 345.7 microg g(-1))相关。由于有利等位基因均来自“OAC Bayfield”，因此可以得出结论，这三个 QTL 对于高 VE 含量的标记辅助选择具有巨大的潜在价值。
