Laboratory of Biochemistry and Environmental Toxicology, ISA, Chott-Mariem, 4042 Sousse, Tunisia.
Ecotoxicol Environ Saf. 2010 Jul;73(5):842-8. doi: 10.1016/j.ecoenv.2009.12.032. Epub 2010 Jan 13.
In the present study, mussel (Mytilus galloprovincialis) digestive gland biotransformation and detoxification responses to acute exposure to the polycyclic aromatic hydrocarbon benzo[a]pyrene (B[a]P) were investigated. Mussels were exposed to a sublethal dose of B[a]P (75 nM; 19 microg/l per animal) for 24, 48 and 72h. The following biological responses were measured in the digestive gland tissues: (1) B[a]P hydroxylase (BPH) activity, as phase I biotransformation parameter; (2) glutathione S-transferase (GST) activity as a phase II conjugation enzyme, (3) catalase (CAT) activity as potential biomarker of oxidative stress, (4) acetylcholinesterase (AChE) activity as an indication of possible neurotoxicity response. DNA damage was assessed over time using the single cell gel electrophoresis comet assay and the micronuclei test. BPH and GST activities showed an increasing trend over exposure period. CAT activity showed a symmetrical bell shape response with a maximum at 48h. AChE activity was significantly depressed after 48 and 72h exposure to B[a]P. Comet assay and micronuclei test in digestive gland cells suggest that B[a]P exposure induced significant DNA damage with a maximum response after 72h exposure.
在本研究中，研究了贻贝（Mytilus galloprovincialis）消化腺对多环芳烃苯并[a]芘（B[a]P）急性暴露的生物转化和解毒反应。贻贝暴露于亚致死剂量的 B[a]P（75 nM；19 微克/升/动物）24、48 和 72 小时。在消化腺组织中测量了以下生物反应：（1）苯并[a]芘羟化酶（BPH）活性，作为 I 相生物转化参数；（2）谷胱甘肽 S-转移酶（GST）活性作为 II 相结合酶，（3）过氧化氢酶（CAT）活性作为氧化应激的潜在生物标志物，（4）乙酰胆碱酯酶（AChE）活性作为可能的神经毒性反应的指示。使用单细胞凝胶电泳彗星试验和微核试验随时间评估 DNA 损伤。BPH 和 GST 活性在暴露期间呈上升趋势。CAT 活性呈对称钟形响应，在 48 小时达到最大值。暴露于 B[a]P 48 和 72 小时后，AChE 活性显著降低。消化腺细胞中的彗星试验和微核试验表明，B[a]P 暴露诱导了显著的 DNA 损伤，在 72 小时暴露后达到最大反应。
