Codexis Inc, 200 Penobscot Drive, Redwood City, CA 94063, USA.
Curr Opin Chem Biol. 2010 Apr;14(2):122-9. doi: 10.1016/j.cbpa.2009.12.003. Epub 2010 Jan 12.
Over the past two years the application of ketoreductases in the commercial synthesis of chiral alcohols has undergone a revolution. Biocatalysts are now often the preferred catalyst for the synthesis of chiral alcohols via ketone reduction and are displacing reagents and chemocatalysts that only recently were considered break-through process solutions themselves. Tailor-made enzymes can now be generated from advanced, non-natural variants using HTP screening and modern molecular biology techniques. At the same time, global economic and environmental pressures direct industrial process development toward versatile platforms that can be applied to the different stages of product development. We will discuss the technologies that have emerged over the past years that have guided biocatalysis from the bottom of the toolbox, to the power tool of choice.
在过去的两年中，酮还原酶在手性醇的商业合成中的应用发生了革命性的变化。生物催化剂现在通常是通过酮还原合成手性醇的首选催化剂，正在取代试剂和化学催化剂，这些试剂和化学催化剂最近才被认为是突破性的工艺解决方案。现在可以使用高通量筛选和现代分子生物学技术，从先进的非天然变体中生成定制酶。与此同时，全球经济和环境压力促使工业过程开发朝着多功能平台发展，该平台可应用于产品开发的不同阶段。我们将讨论过去几年出现的技术，这些技术将生物催化从工具箱的底部引导到首选的电动工具。
