粪便标本中艰难梭菌 027 型 PCR 核糖体分型多位点可变数目串联重复分析亚型的共存。
Coexistence of multiple multilocus variable-number tandem-repeat analysis subtypes of Clostridium difficile PCR ribotype 027 strains within fecal specimens.
机构信息
Health Protection Agency, West Midlands Public Health Laboratory, Heart of England NHS Foundation Trust, Birmingham B9 5SS, United Kingdom.
出版信息
J Clin Microbiol. 2010 Mar;48(3):985-7. doi: 10.1128/JCM.02012-09. Epub 2010 Jan 13.
Abstract
We investigated whether multilocus variable-number tandem-repeat analysis (MLVA) typing could identify different subtypes of Clostridium difficile ribotype 027 within the same feces specimen. Five of 39 specimens yielded at least one isolate with an MLVA profile different (more than five summed tandem repeat differences) from that of other isolates in the same specimen, thereby potentially obscuring epidemiological links between C. difficile infection cases.
摘要
我们研究了多位点可变数目串联重复分析（MLVA）分型是否可以在同一粪便标本中识别出不同亚型的艰难梭菌 027 型。在 39 个标本中有 5 个至少有一个分离株的 MLVA 图谱与同一标本中的其他分离株不同（多于五个串联重复差异），从而可能掩盖艰难梭菌感染病例之间的流行病学联系。
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