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粪便标本中艰难梭菌 027 型 PCR 核糖体分型多位点可变数目串联重复分析亚型的共存。

Coexistence of multiple multilocus variable-number tandem-repeat analysis subtypes of Clostridium difficile PCR ribotype 027 strains within fecal specimens.

机构信息

Health Protection Agency, West Midlands Public Health Laboratory, Heart of England NHS Foundation Trust, Birmingham B9 5SS, United Kingdom.

出版信息

J Clin Microbiol. 2010 Mar;48(3):985-7. doi: 10.1128/JCM.02012-09. Epub 2010 Jan 13.

DOI:10.1128/JCM.02012-09
PMID:20071546
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2832431/
Abstract

We investigated whether multilocus variable-number tandem-repeat analysis (MLVA) typing could identify different subtypes of Clostridium difficile ribotype 027 within the same feces specimen. Five of 39 specimens yielded at least one isolate with an MLVA profile different (more than five summed tandem repeat differences) from that of other isolates in the same specimen, thereby potentially obscuring epidemiological links between C. difficile infection cases.

摘要

我们研究了多位点可变数目串联重复分析（MLVA）分型是否可以在同一粪便标本中识别出不同亚型的艰难梭菌 027 型。在 39 个标本中有 5 个至少有一个分离株的 MLVA 图谱与同一标本中的其他分离株不同（多于五个串联重复差异），从而可能掩盖艰难梭菌感染病例之间的流行病学联系。

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Coexistence of multiple multilocus variable-number tandem-repeat analysis subtypes of Clostridium difficile PCR ribotype 027 strains within fecal specimens.粪便标本中艰难梭菌 027 型 PCR 核糖体分型多位点可变数目串联重复分析亚型的共存。
J Clin Microbiol. 2010 Mar;48(3):985-7. doi: 10.1128/JCM.02012-09. Epub 2010 Jan 13.
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Infect Genet Evol. 2009 Dec;9(6):1410-7. doi: 10.1016/j.meegid.2009.06.005. Epub 2009 Jun 16.
2
Application of multiple-locus variable-number tandem-repeat analysis to determine clonal spread of toxin A-negative Clostridium difficile in a general hospital in Buenos Aires, Argentina.多基因座可变数目串联重复分析在阿根廷布宜诺斯艾利斯一家综合医院中用于确定产毒阴性艰难梭菌的克隆传播。
Clin Microbiol Infect. 2009 Dec;15(12):1080-6. doi: 10.1111/j.1469-0691.2009.02759.x. Epub 2009 May 11.
3
Clostridium difficile PCR ribotype 078 toxinotype V found in diarrhoeal pigs identical to isolates from affected humans.在腹泻猪中发现的艰难梭菌PCR核糖体分型078毒素型V与来自受影响人类的分离株相同。
Environ Microbiol. 2009 Feb;11(2):505-11. doi: 10.1111/j.1462-2920.2008.01790.x.
4
Update of Clostridium difficile infection due to PCR ribotype 027 in Europe, 2008.2008年欧洲艰难梭菌PCR核糖体分型027感染的最新情况
Euro Surveill. 2008 Jul 31;13(31):18942.
5
Clindamycin-resistant clone of Clostridium difficile PCR Ribotype 027, Europe.欧洲艰难梭菌PCR核糖型027的克林霉素耐药克隆
Emerg Infect Dis. 2008 Sep;14(9):1485-7. doi: 10.3201/eid1409.071346.
6
Emergence of reduced susceptibility to metronidazole in Clostridium difficile.艰难梭菌对甲硝唑敏感性降低的出现。
J Antimicrob Chemother. 2008 Nov;62(5):1046-52. doi: 10.1093/jac/dkn313. Epub 2008 Aug 7.
7
Use of highly discriminatory fingerprinting to analyze clusters of Clostridium difficile infection cases due to epidemic ribotype 027 strains.使用高分辨率指纹图谱分析由流行核糖型027菌株引起的艰难梭菌感染病例群。
J Clin Microbiol. 2008 Mar;46(3):954-60. doi: 10.1128/JCM.01764-07. Epub 2008 Jan 23.
8
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Euro Surveill. 2007 Apr 26;12(4):E070426.2. doi: 10.2807/esw.12.17.03182-en.
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Typing and subtyping of Clostridium difficile isolates by using multiple-locus variable-number tandem-repeat analysis.运用多位点可变数目串联重复序列分析对艰难梭菌分离株进行分型和亚型分析。
J Clin Microbiol. 2007 Mar;45(3):1024-8. doi: 10.1128/JCM.02023-06. Epub 2006 Dec 13.