光介导的空间控制通过光不稳定荧光猝灭肽盒。
Light-mediated spatial control via photolabile fluorescently quenched peptide cassettes.
机构信息
Department of Chemistry, Division of Medicinal Chemistry and Natural Products, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
出版信息
J Am Chem Soc. 2010 Feb 10;132(5):1446-7. doi: 10.1021/ja907427p.
Abstract
Light-regulatable compounds are finding increasing utility as spatial and temporal probes of biological behavior. An independent measure of successful light-induced structural change is possible when alteration (e.g., activation, deactivation, etc.) of the bioprobe can be directly linked to a fluorescent readout. We have identified a series of photolabile fluorescently quenched cassettes that display extraordinarily large fluorescence enhancements upon photolysis. A pair of cassettes has been inserted into mitochondrial localization sequences to assess an organelle-targeted light-mediated release strategy for controlling biological activity. The peptide constructs are readily absorbed by mitochondria and subsequently can be cleaved in a light-dependent fashion as assessed by the predicted changes in absorbance and fluorescence.
摘要
光调控化合物作为生物行为的时空探针越来越受到重视。当生物探针的改变（例如激活、失活等）可以直接与荧光读数相关联时，就有可能对成功的光诱导结构变化进行独立测量。我们已经确定了一系列光不稳定的荧光猝灭盒，它们在光解时显示出非常大的荧光增强。一对盒已被插入线粒体定位序列中，以评估用于控制生物活性的细胞器靶向光介导释放策略。肽结构很容易被线粒体吸收，随后可以通过预测的吸光度和荧光变化来以光依赖的方式进行切割。
相似文献
1
Light-mediated spatial control via photolabile fluorescently quenched peptide cassettes.光介导的空间控制通过光不稳定荧光猝灭肽盒。
J Am Chem Soc. 2010 Feb 10;132(5):1446-7. doi: 10.1021/ja907427p.
2
Effects of photolabile calcium chelators on fluorescent calcium indicators.光不稳定钙螯合剂对荧光钙指示剂的影响。
Cell Calcium. 1992 Jan;13(1):29-40. doi: 10.1016/0143-4160(92)90027-p.
3
Proteolytic fingerprinting of complex biological samples using combinatorial libraries of fluorogenic probes.使用荧光探针组合文库对复杂生物样品进行蛋白水解指纹分析。
Curr Protoc Protein Sci. 2012 Nov;Chapter 21:21.22.1-21.22.14. doi: 10.1002/0471140864.ps2122s70.
4
Caged mono- and divalent ligands for light-assisted disruption of PDZ domain-mediated interactions.笼状单原子和二价配体用于光辅助破坏 PDZ 结构域介导的相互作用。
J Am Chem Soc. 2013 Mar 27;135(12):4580-3. doi: 10.1021/ja309870q. Epub 2013 Mar 13.
5
Photodamage of lipid bilayers by irradiation of a fluorescently labeled cell-penetrating peptide.通过照射荧光标记的细胞穿透肽对脂质双层造成的光损伤。
Biochim Biophys Acta. 2014 Jan;1840(1):507-15. doi: 10.1016/j.bbagen.2013.10.011. Epub 2013 Oct 14.
6
Visual detection of specific, native interactions between soluble and microbead-tethered alpha-helices from membrane proteins.对来自膜蛋白的可溶性α螺旋与微珠连接的α螺旋之间特定的天然相互作用进行视觉检测。
Biochemistry. 2001 Nov 20;40(46):13753-9. doi: 10.1021/bi011449n.
7
Masked rhodamine dyes of five principal colors revealed by photolysis of a 2-diazo-1-indanone caging group: synthesis, photophysics, and light microscopy applications.通过2-重氮基-1-茚满酮笼蔽基团光解揭示的五种主要颜色的掩蔽罗丹明染料：合成、光物理性质及光学显微镜应用
Chemistry. 2014 Oct 6;20(41):13162-73. doi: 10.1002/chem.201403316. Epub 2014 Sep 5.
8
Lipid pools as photolabile "protecting groups": design of light-activatable bioagents.脂质池作为光不稳定的“保护基团”：光活化生物试剂的设计。
Angew Chem Int Ed Engl. 2013 Sep 16;52(38):9936-9. doi: 10.1002/anie.201305510. Epub 2013 Jul 31.
9
Interaction of heparin with internally quenched fluorogenic peptides derived from heparin-binding consensus sequences, kallistatin and anti-thrombin III.肝素与源自肝素结合共有序列、激肽释放酶抑制蛋白和抗凝血酶III的内部淬灭荧光肽的相互作用。
Biochem J. 2002 Sep 1;366(Pt 2):435-46. doi: 10.1042/BJ20020023.
10
Peptide-Induced AIEgen Self-Assembly: A New Strategy to Realize Highly Sensitive Fluorescent Light-Up Probes.肽诱导的 AIE 分子自组装：实现高灵敏度荧光点亮探针的新策略。
Anal Chem. 2016 Apr 5;88(7):3872-8. doi: 10.1021/acs.analchem.6b00023. Epub 2016 Mar 16.
引用本文的文献
1
Vitamin B12: a tunable, long wavelength, light-responsive platform for launching therapeutic agents.维生素B12：一个用于递送治疗剂的可调节的长波长光响应平台。
Acc Chem Res. 2015 Nov 17;48(11):2866-74. doi: 10.1021/acs.accounts.5b00331. Epub 2015 Oct 19.
2
Merging of confocal and caging technologies: selective three-color communication with profluorescent reporters.共聚焦和光笼技术的融合：具有前荧光报告基团的选择性三颜色通讯。
Angew Chem Int Ed Engl. 2012 Jul 27;51(31):7684-7. doi: 10.1002/anie.201202820. Epub 2012 Jun 27.
3
Construction of a photoactivatable profluorescent enzyme via propinquity labeling.通过邻近标记构建光激活的前荧光酶。
J Am Chem Soc. 2011 Mar 2;133(8):2331-3. doi: 10.1021/ja108950q. Epub 2011 Feb 8.
4
Dual wavelength photoactivation of cAMP- and cGMP-dependent protein kinase signaling pathways.双波长光激活 cAMP 和 cGMP 依赖的蛋白激酶信号通路。
ACS Chem Biol. 2011 Apr 15;6(4):377-84. doi: 10.1021/cb100398e. Epub 2011 Jan 26.
5
Photochemical cleavage of leader peptides.导肽的光化学切割。
Chem Commun (Camb). 2010 Dec 21;46(47):8935-7. doi: 10.1039/c0cc02945a. Epub 2010 Nov 2.
6
Suborganelle sensing of mitochondrial cAMP-dependent protein kinase activity.线粒体 cAMP 依赖性蛋白激酶活性的亚细胞器感应。
J Am Chem Soc. 2010 May 5;132(17):6075-80. doi: 10.1021/ja909652q.
本文引用的文献
1
Uber-responsive peptide-based sensors of signaling proteins.基于肽的信号蛋白超灵敏传感器。
Angew Chem Int Ed Engl. 2009;48(40):7290-2. doi: 10.1002/anie.200903717.
2
Targeting mitochondria with organelle-specific compounds: strategies and applications.利用细胞器特异性化合物靶向线粒体：策略与应用
Chembiochem. 2009 Aug 17;10(12):1939-50. doi: 10.1002/cbic.200900185.
3
Illuminating the chemistry of life: design, synthesis, and applications of "caged" and related photoresponsive compounds.揭示生命化学：“笼形”及相关光响应化合物的设计、合成与应用
ACS Chem Biol. 2009 Jun 19;4(6):409-27. doi: 10.1021/cb900036s.
4
Mitochondria-penetrating peptides.线粒体穿透肽
Chem Biol. 2008 Apr;15(4):375-82. doi: 10.1016/j.chembiol.2008.03.015.
5
Biologically active molecules with a "light switch".带有“光开关”的生物活性分子。
Angew Chem Int Ed Engl. 2006 Jul 24;45(30):4900-21. doi: 10.1002/anie.200600387.
6
A close look at fluorescence quenching of organic dyes by tryptophan.深入研究色氨酸对有机染料的荧光猝灭作用。
Chemphyschem. 2005 Nov 11;6(11):2277-85. doi: 10.1002/cphc.200500191.
7
Release and report: a new photolabile caging system with a two-photon fluorescence reporting function.释放与报告：一种具有双光子荧光报告功能的新型光不稳定笼化系统。
J Am Chem Soc. 2005 Sep 14;127(36):12458-9. doi: 10.1021/ja053654m.
8
Simultaneous triggering of protein activity and fluorescence.蛋白质活性与荧光的同步触发。
J Am Chem Soc. 2004 Jun 16;126(23):7170-1. doi: 10.1021/ja0499142.
9
Advances in the analysis of single mitochondria.
Curr Opin Biotechnol. 2003 Feb;14(1):35-41. doi: 10.1016/s0958-1669(02)00008-3.
10
NH2-Terminal targeting motifs direct dual specificity A-kinase-anchoring protein 1 (D-AKAP1) to either mitochondria or endoplasmic reticulum.氨基末端靶向基序将双特异性A激酶锚定蛋白1（D-AKAP1）导向线粒体或内质网。
J Cell Biol. 1999 May 31;145(5):951-9. doi: 10.1083/jcb.145.5.951.
Zotero 插件