Department of Chemistry, Division of Medicinal Chemistry and Natural Products, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
J Am Chem Soc. 2010 Feb 10;132(5):1446-7. doi: 10.1021/ja907427p.
Light-regulatable compounds are finding increasing utility as spatial and temporal probes of biological behavior. An independent measure of successful light-induced structural change is possible when alteration (e.g., activation, deactivation, etc.) of the bioprobe can be directly linked to a fluorescent readout. We have identified a series of photolabile fluorescently quenched cassettes that display extraordinarily large fluorescence enhancements upon photolysis. A pair of cassettes has been inserted into mitochondrial localization sequences to assess an organelle-targeted light-mediated release strategy for controlling biological activity. The peptide constructs are readily absorbed by mitochondria and subsequently can be cleaved in a light-dependent fashion as assessed by the predicted changes in absorbance and fluorescence.
光调控化合物作为生物行为的时空探针越来越受到重视。当生物探针的改变（例如激活、失活等）可以直接与荧光读数相关联时，就有可能对成功的光诱导结构变化进行独立测量。我们已经确定了一系列光不稳定的荧光猝灭盒，它们在光解时显示出非常大的荧光增强。一对盒已被插入线粒体定位序列中，以评估用于控制生物活性的细胞器靶向光介导释放策略。肽结构很容易被线粒体吸收，随后可以通过预测的吸光度和荧光变化来以光依赖的方式进行切割。
