Kubota K, Yamada S, Ito M, Nakamura W, Nagayo T
J Natl Cancer Inst. 1977 Dec;59(6):1599-604. doi: 10.1093/jnci/59.6.1599.
Histochemical studies were performed on signet-ring cells in surgically resected gastric carcinomas from 64 patients. On the basis of periodic acid-Schiff reactivity of the intracellular mucin, the signet-ring cells were classified as type A (immature), type B (intermediate), or type C (mature). Different staining reactions to Alcian blue at pH 2.5 enabled type C cells to be subclassified as C-1 or C-2. In 63 cases (98.4%), intense leucine naphthylamidase (LNAase) activity was found histochemically in the cytoplasm of nearly all cancer cells except type C-2. Localization of LNAase activity in the cytoplasm was confirmed by conventional biochemical assay. From the present results, histochemical characteristics of signet-ring cell carcinoma are discussed.
对64例手术切除的胃癌印戒细胞进行了组织化学研究。根据细胞内黏液的过碘酸-希夫反应性，将印戒细胞分为A 型（不成熟型）、B型（中间型）或C型（成熟型）。在pH 2.5条件下对阿尔辛蓝有不同的染色反应，使得C型细胞可进一步分为C-1或C-2亚型。在63例（98.4%）病例中，通过组织化学方法在几乎所有癌细胞（C-2型除外）的细胞质中发现了强烈的亮氨酸萘基酰胺酶（LNAase）活性。通过传统生化分析证实了LNAase活性在细胞质中的定位。根据目前的结果，对印戒细胞癌的组织化学特征进行了讨论。
