Tatematsu M, Furihata C, Katsuyama T, Miki K, Honda H, Konishi Y, Ito N
Cancer Res. 1986 Sep;46(9):4866-72.
Gastric and intestinal phenotypic expression in 37 surgically obtained primary signet ring cell carcinomas, five of their metastases to lymph nodes, and three signet ring cell carcinomas transplanted into nude mice were determined by biochemical, mucin, histochemical, and ultrastructural studies. Crude extracts of cancer tissues were used for measurements of pepsinogen isozymes, sucrase, aminopeptidase (microsomal), and alkaline phosphatase. Histochemical staining of mucin by paradoxical concanavalin A, the galactose oxidase-Schiff sequence and sialidase-galactose oxidase-Schiff, and the periodate-borohydride technique/potassium hydroxide/periodic acid-Schiff procedure was performed. The procedures allowed clear definition of pyloric gland, surface mucous, small and large intestinal goblet, and intestinal absorptive cell types. Of 40 specimens examined, 19 consisted entirely of gastric-type cells, and three entirely of intestinal-type cells. The others consisted of mixtures of gastric and intestinal-type cells. The observed high incidence of intestinal-type cells in signet ring cell carcinomas suggested that intestinal-type cells develop independently from intestinal metaplasia within signet ring cell carcinomas (diffuse-type gastric cancers), which probably originate from nonmetaplastic gastric mucosa.
通过生化、黏液、组织化学和超微结构研究，确定了37例手术获取的原发性印戒细胞癌、其中5例转移至淋巴结的标本以及3例移植到裸鼠体内的印戒细胞癌的胃和肠表型表达。癌组织的粗提物用于检测胃蛋白酶原同工酶、蔗糖酶、氨肽酶（微粒体）和碱性磷酸酶。采用伴刀豆球蛋白A、半乳糖氧化酶-席夫序列和唾液酸酶-半乳糖氧化酶-席夫以及高碘酸盐-硼氢化物技术/氢氧化钾/高碘酸-席夫程序对黏液进行组织化学染色。这些程序能够清晰地界定幽门腺、表面黏液、小肠和大肠杯状以及肠吸收细胞类型。在检查的40个标本中，19个完全由胃型细胞组成，3个完全由肠型细胞组成。其他标本则由胃型和肠型细胞混合组成。在印戒细胞癌中观察到的肠型细胞的高发生率表明，肠型细胞在印戒细胞癌（弥漫型胃癌）中独立于肠化生发展而来，而印戒细胞癌可能起源于未化生的胃黏膜。
