通过酶联免疫吸附测定法检测治疗对微小膜壳绦虫血清抗体的影响。
Effect of treatment on serum antibody to Hymenolepis nana detected by enzyme-linked immunosorbent assay.
作者信息
Castillo R M, Grados P, Carcamo C, Miranda E, Montenegro T, Guevara A, Gilman R H
机构信息
Department of Microbiology, Cayetano Heredia University, Lima, Peru.
出版信息
J Clin Microbiol. 1991 Feb;29(2):413-4. doi: 10.1128/jcm.29.2.413-414.1991.
An enzyme-linked immunosorbent assay (ELISA) was developed to measure serum immunoglobulin G antibodies in 65 patients infected with Hymenolepis nana and 30 noninfected patients. Antibody was detected in 51 of 65 (sensitivity, 79%) and 5 of 30 H. nana-negative patients (specificity, 83%). Nine patients infected with H. nana were treated with praziquantel (20 to 25 mg/kg of body weight). Antibody disappeared from the sera at 90 days in six patients, five of whom had eliminated H. nana. Antibody persisted in three patients in whom H. nana infection did not clear after treatment. The H. nana ELISA had a high rate of cross-reactions with sera from patients with cysticercosis (8 of 29 [28%]) and hydatidosis (8 of 23 [35%]). The ELISA for H. nana may be useful for defining the epidemiology of H. nana infections, especially in areas free from cysticercosis and hydatidosis.
开发了一种酶联免疫吸附测定法（ELISA），用于检测65例感染微小膜壳绦虫的患者和30例未感染患者的血清免疫球蛋白G抗体。在65例患者中的51例（敏感性为79%）以及30例微小膜壳绦虫阴性患者中的5例（特异性为83%）检测到了抗体。9例感染微小膜壳绦虫的患者接受了吡喹酮治疗（20至25毫克/千克体重）。6例患者在90天时血清中的抗体消失，其中5例已清除微小膜壳绦虫。3例患者治疗后微小膜壳绦虫感染未清除，其抗体持续存在。微小膜壳绦虫ELISA与囊尾蚴病患者血清（29例中的8例[28%]）和包虫病患者血清（23例中的8例[35%]）有较高的交叉反应率。微小膜壳绦虫ELISA可能有助于确定微小膜壳绦虫感染的流行病学情况，尤其是在无囊尾蚴病和包虫病的地区。
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