Heath D D, Lawrence S B, Glennie A, Twaalfhoven H
J Parasitol. 1985 Apr;71(2):192-9.
The excretory and secretory antigens from the evaginated scoleces of Taenia ovis were collected for 3 days in vitro, and used in an ELISA test to detect antibodies to T. ovis in the serum of dogs. When tested on sequentially collected sera, diagnostic ELISA values could be detected in many dogs 4 wk after infection, and remained for an average of a further 4 wk after worms were removed from dogs with an anthelmintic. Using an ELISA discriminant value that eliminated all false positives from 70 uninfected laboratory dog sera and from 57 uninfected farm dog sera, 54/62 true positives were found in sera from dogs infected with various numbers of T. ovis for various intervals. Sera from dogs infected with T. hydatigena gave 11/15 false positive reactions, whereas sera from 15 dogs infected with Echinococcus granulosus or 7 dogs infected with T. pisiformis were all negative. For T. ovis the test had a high repeatability, was not greatly influenced by the number of worms carried by the dog and higher titres were correlated with long-standing infections. Approximately 1,000 scoleces could be recovered from each experimentally infected sheep. Using the ELISA test with undiluted antigen and serum diluted 1:40, approximately 10 sera could be tested in duplicate with the excretions and secretions from each T. ovis scolex.
收集绵羊带绦虫外翻头节的排泄和分泌抗原，在体外培养3天，用于酶联免疫吸附测定（ELISA）检测犬血清中抗绵羊带绦虫抗体。对连续采集的血清进行检测时，许多犬在感染后4周可检测到诊断性ELISA值，在用驱虫药从犬体内清除虫体后，该值平均还会持续4周。使用一个ELISA判别值，该值可消除70只未感染实验犬血清和57只未感染农场犬血清中的所有假阳性，在感染不同数量绵羊带绦虫不同时间的犬血清中，发现54/62个真阳性。感染泡状带绦虫的犬血清出现11/15个假阳性反应，而15只感染细粒棘球绦虫的犬血清或7只感染豆状带绦虫的犬血清均为阴性。对于绵羊带绦虫，该检测具有高重复性，受犬体内虫体数量影响不大，且较高滴度与长期感染相关。从每只经实验感染的绵羊体内可回收约1000个头节。使用未稀释抗原和1:40稀释血清的ELISA检测，用每个绵羊带绦虫头节的排泄和分泌物可对约10份血清进行重复检测。
