Department of Pharmaceutical Chemistry & Bioanalytics, Institute of Pharmacy, Martin-Luther-Universität Halle-Wittenberg, Wolfgang-Langenbeck-Strasse 4, 06120 Halle (Saale), Germany.
Anal Bioanal Chem. 2010 Aug;397(8):3433-40. doi: 10.1007/s00216-009-3405-5. Epub 2010 Jan 15.
The identification of protein-protein interactions within their physiological environment is the key to understanding biological processes at the molecular level. However, the artificial nature of in vitro experiments, with their lack of other cellular components, may obstruct observations of specific cellular processes. In vivo analyses can provide information on the processes within a cell that might not be observed in vitro. Chemical crosslinking combined with mass spectrometric analysis of the covalently connected binding partners allows us to identify interacting proteins and to map their interface regions directly in the cell. In this paper, different in vivo crosslinking strategies for deriving information on protein-protein interactions in their physiological environment are described.
在生理环境中鉴定蛋白质-蛋白质相互作用是理解分子水平生物过程的关键。然而，体外实验的人为性质及其缺乏其他细胞成分，可能会阻碍对特定细胞过程的观察。体内分析可以提供关于细胞内过程的信息，这些信息在体外可能观察不到。化学交联结合对共价连接的结合伴侣进行质谱分析，使我们能够直接在细胞中鉴定相互作用的蛋白质并绘制它们的界面区域。本文描述了不同的体内交联策略，用于获取生理环境中蛋白质-蛋白质相互作用的信息。
