Singh Ravinder J
Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.
Methods Mol Biol. 2010;603:509-17. doi: 10.1007/978-1-60761-459-3_50.
25-OH-vitamin D (25OHD) is ordered and interpreted clinically in light of calcium balance and homeostasis. Various methodologies including immunoassay and chromatography are described for the measurement of 25OHD in biological fluids. It is well reported that all existing methods are challenging and require a high level of technical expertise. While this is also true of the methods using liquid chromatography-tandem mass spectrometry (LC-MS-MS), the technique is gaining favor in various laboratories because it offers advantages of greatly improved specificity and sensitivity.
25-羟基维生素D（25OHD）是根据钙平衡和内稳态进行临床检测和解读的。描述了包括免疫测定和色谱法在内的各种用于测量生物体液中25OHD的方法。有充分报道称，所有现有方法都具有挑战性，需要高水平的技术专长。虽然使用液相色谱-串联质谱法（LC-MS-MS）的方法也是如此，但该技术在各个实验室中越来越受欢迎，因为它具有特异性和灵敏度大大提高的优点。
