应用液相色谱-串联质谱法对糖尿病患者血清 25(OH)D2 和 25(OH)D3 浓度进行定量分析。
Quantitation of serum 25(OH)D2 and 25(OH)D3 concentrations by liquid chromatography tandem mass spectrometry in patients with diabetes mellitus.
机构信息
Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.
Department of Laboratory Medicine, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
出版信息
J Food Drug Anal. 2019 Apr;27(2):510-517. doi: 10.1016/j.jfda.2018.12.004. Epub 2018 Dec 31.
Vitamin D has been considered to regulate calcium and phosphorus homeostasis and to preserve skeletal integrity. Serum 25-hydroxyvitamin D (25(OH)D) is the best indicator of vitamin D levels. The association of serum 25(OH)D deficiency with increased risk of type 1 diabetes (T1DM) and type 2 diabetes (T2DM) is controversial. We investigated serum 25(OH)D and 25(OH)D levels in diabetes patients by using liquid chromatography tandem mass spectrometry (LC-MS/MS). Serum 25(OH)D2 and 25(OH)D3 levels were measured with liquid chromatography tandem mass spectrometry in electrospray ionization positive mode. Chromatograms were separated using an ACE5 C18 column on a gradient of methanol. The total 25(OH)D levels were calculated as the sum of 25(OH)D3 and 25(OH)D2 levels. A total of 56 patients with T1DM and 41 patients with T2DM were enrolled in this study. There were 42 and 28 non-diabetic, age-matched volunteers who participated as the T1DM controls and the T2DM controls, respectively. The total 25(OH)D levels were lowest in the 21-40 age group. The levels of both 25(OH)D3 and the total 25(OH)D were significantly higher in the T1DM and T2DM groups than in the controls (p < 0.01 in T1DM and p < 0.05 in T2DM group, respectively). The 25(OH)D2 levels were only significantly higher in T1DM patients than in the controls. The percentages of vitamin D deficiency (total 25(OH)D less than 20 ng/mL) in the T1DM, T2DM, the T1DM controls and the T2DM controls were 7.1%, 0%, 14.3% and 3.6%, respectively. The percentages of vitamin D insufficiency (total 25(OH)D less than 30 ng/mL) in the T1DM, T2DM, the T1DM controls and the T2DM controls were 26.8%, 7.3%, 54.8% and 17.9%, respectively. The percentages of vitamin D deficiency and insufficiency were significantly lower in the T1DM patients than in the T1DM controls (p < 0.01). In the present study, both type 1 and type 2 diabetes patients had higher serum 25(OH)D levels and lower percentages of vitamin D deficiency/insufficiency.
维生素 D 被认为可以调节钙磷平衡，维持骨骼完整性。血清 25-羟维生素 D（25（OH）D）是维生素 D 水平的最佳指标。血清 25（OH）D 缺乏与 1 型糖尿病（T1DM）和 2 型糖尿病（T2DM）风险增加之间的关联存在争议。我们使用液相色谱串联质谱法（LC-MS/MS）检测糖尿病患者的血清 25（OH）D。采用电喷雾正离子模式下的液相色谱串联质谱法测定血清 25（OH）D2 和 25（OH）D3 水平。采用甲醇梯度洗脱的 ACE5 C18 柱分离色谱图。总 25（OH）D 水平计算为 25（OH）D3 和 25（OH）D2 水平的总和。本研究共纳入 56 例 T1DM 患者和 41 例 T2DM 患者。42 名和 28 名年龄匹配的非糖尿病志愿者分别作为 T1DM 对照组和 T2DM 对照组参与研究。总 25（OH）D 水平在 21-40 岁年龄组最低。T1DM 和 T2DM 组的血清 25（OH）D3 和总 25（OH）D 水平均明显高于对照组（T1DM 组 p<0.01，T2DM 组 p<0.05）。仅 T1DM 患者的 25（OH）D2 水平明显高于对照组。T1DM、T2DM、T1DM 对照组和 T2DM 对照组维生素 D 缺乏（总 25（OH）D<20ng/ml）的百分比分别为 7.1%、0%、14.3%和 3.6%。T1DM、T2DM、T1DM 对照组和 T2DM 对照组维生素 D 不足（总 25（OH）D<30ng/ml）的百分比分别为 26.8%、7.3%、54.8%和 17.9%。T1DM 患者的维生素 D 缺乏和不足的百分比明显低于 T1DM 对照组（p<0.01）。在本研究中，1 型和 2 型糖尿病患者的血清 25（OH）D 水平较高，维生素 D 缺乏/不足的百分比较低。
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