Department of Pharmaceutical and Biological Chemistry, Centre for Pharmacognosy and Phytotherapy, School of Pharmacy, University of London, London WC1N 1AX, UK.
Phytother Res. 2010 Jul;24(7):1099-103. doi: 10.1002/ptr.3094.
As part of our continuing research on seaweeds, we have screened the crude extracts of 23 red marine algae collected from England and Ireland. The clinically important blood-stage life forms of Trypanosoma brucei rhodesiense, T. cruzi, Leishmania donovani and Mycobacterium tuberculosis were used as test organisms in the in vitro assays. The selectivity of the extracts was determined by using mammalian skeletal myoblast (L6) cells. All algal extracts showed activity against T. brucei rhodesiense, with Corallina officinalis and Ceramium virgatum being the most potent (IC(50) values 4.8 and 5.4 microg/ml), whilst none of the algal extracts inhibited the growth of T. cruzi. Except for Porphyra leucosticta, extracts from all seaweeds also showed leishmanicidal activity with IC(50) values ranging from 16.5 to 85.6 microg/ml. Only the crude extract of Calliblepharis jubata showed some weak activity against Mycobacterium tuberculosis (MIC value 256 microg/ml), while the others were inactive at this concentration. Corallina officinalis was the only seaweed that displayed some marginal cytotoxicity (IC(50) value 88.6 microg/ml), and all remaining extracts were non-toxic towards L6 cells at 90 microg/ml concentration. To our knowledge, this is the first study reporting antiprotozoal and antimycobacterial activity of British and Irish red algae.
作为我们对海藻持续研究的一部分，我们已经筛选了来自英国和爱尔兰的 23 种红海藻的粗提取物。临床上重要的布氏锥虫罗得西亚期、克氏锥虫、利什曼原虫和结核分枝杆菌的血期生活形式被用作体外测定中的测试生物。提取物的选择性通过使用哺乳动物骨骼肌成肌细胞（L6）细胞来确定。所有藻类提取物均显示出对布氏锥虫罗得西亚期的活性，其中珊瑚藻和角叉菜最为有效（IC50 值分别为 4.8 和 5.4μg/ml），而没有一种藻类提取物抑制克氏锥虫的生长。除了边缘马尾藻外，所有海藻的提取物也显示出杀利什曼原虫活性，IC50 值范围为 16.5 至 85.6μg/ml。只有 Calliblepharis jubata 的粗提取物对结核分枝杆菌显示出一些较弱的活性（MIC 值为 256μg/ml），而其他在该浓度下均无活性。珊瑚藻是唯一显示出一些边缘细胞毒性的海藻（IC50 值为 88.6μg/ml），所有剩余的提取物在 90μg/ml 浓度下对 L6 细胞均无毒性。据我们所知，这是首次报道英国和爱尔兰红海藻具有抗原生动物和抗分枝杆菌活性的研究。
