Department of Pharmaceutical and Biological Chemistry, Centre for Pharmacognosy and Phytotherapy, School of Pharmacy, University of London, London WC1N 1AX, UK.
Phytother Res. 2010 Nov;24(11):1724-9. doi: 10.1002/ptr.3208.
In the continuation of our research on seaweeds, crude extracts of 21 brown algae collected from the south coast of England and the west coast of Ireland were screened for in vitro trypanocidal, leishmanicidal and antimycobacterial activities. Mammalian stages of a small set of parasitic protozoa; i.e. Trypanosoma brucei rhodesiense, T. cruzi and Leishmania donovani, and the tubercle bacillus Mycobacterium tuberculosis were used as test organisms. The extracts were also evaluated for selectivity by testing on a mammalian cell line (L6 cells). Only four extracts were moderately active against T. cruzi, whereas all algal extracts showed significant activity against T. brucei rhodesiense, with Halidrys siliquosa and Bifurcaria bifurcata (Sargassaceae) being the most potent (IC50 values 1.2 and 1.9 μg/mL). All algal extracts also displayed leishmanicidal activity, with H. siliquosa and B. bifurcata again being the most active (IC50s 6.4 and 8.6 μg/mL). When tested against M. tuberculosis, only the B. bifurcata extract was found to have some antitubercular potential (MIC value 64.0 μg/mL). Only three seaweed extracts, i.e. H. siliquosa, B. bifurcata and Cystoseira tamariscifolia showed some cytotoxicity. To our knowledge, this is the first study on the antiprotozoal and antimycobacterial activity of brown algae from British and Irish waters.
在我们对海藻的研究继续进行的过程中，我们从英国南部海岸和爱尔兰西海岸采集了 21 种褐藻的粗提取物，对其进行了体外抗锥虫、抗利什曼原虫和抗分枝杆菌活性筛选。我们使用了一组小型寄生原生动物的哺乳动物阶段，即非洲锥虫罗得西亚株（Trypanosoma brucei rhodesiense）、克氏锥虫（T. cruzi）和杜氏利什曼原虫（Leishmania donovani），以及结核分枝杆菌（Mycobacterium tuberculosis）作为测试生物。还通过在哺乳动物细胞系（L6 细胞）上进行测试来评估提取物的选择性。只有四种提取物对 T. cruzi 具有中度活性，而所有藻类提取物对 T. brucei rhodesiense 均表现出显著的活性，其中石莼（Halidrys siliquosa）和二叉藻（Bifurcaria bifurcata）（马尾藻科）最为有效（IC50 值分别为 1.2 和 1.9 μg/mL）。所有藻类提取物也表现出抗利什曼原虫活性，石莼和二叉藻再次表现出最强的活性（IC50 值分别为 6.4 和 8.6 μg/mL）。当测试对结核分枝杆菌的活性时，只有二叉藻提取物被发现具有一定的抗结核潜力（MIC 值为 64.0 μg/mL）。只有三种海藻提取物，即石莼、二叉藻和马尾藻（Cystoseira tamariscifolia）表现出一定的细胞毒性。据我们所知，这是首次对来自英国和爱尔兰水域的褐藻的抗原生动物和抗分枝杆菌活性进行研究。
