He Ya-Qing, Zhang Hong-Bin, Yao Xiang-Jie, Liao Yu-Xue, Yang Hong, Xian Hui-Xia, Yang Fan, Zhang Hai-Long, Yang Xiao-Ke, Xu Wen-Bo
Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2011 Jun;25(3):173-5.
Genetic evolution of VP1 of enterovirus type 71 in Shenzhen were analyzed.
All samples were tested by RT-PCR using EV71 specific primer. The VP1 of EV71 were amplified and sequenced. A phylogenetic tree was constructed by comparison of the sequences with subgenotype A, B and C using DNAStar, BioEdit and Mega 3.1 software.
Among 35 strains, the homogeneity of the VP1 nucleotide sequence was between 92.1%-100%. The homogeneity of the VP1 nucleotide sequence with subgenotype A and B was between 81.4% -91.1%. The VP1 nucleotide sequence of 35 strains of Shenzhen shared between 93% -97.4% identity with cluster C4. The prevalence strains of EV71 were cluster C4b from 1998 to 2004, and gradually moved to C4a since 2003. All of EV71 were C4b from 2006 to 2008. Also, the homogeneity of the VP1 nucleotide sequence with Anhui FY23 EV71 strain were 94.5% -94.7%, 95.7% -95.8%, 96.2%, 95.4% -97.5%, 96.3% -99.2% from 2003 to 2008. It shows that the homogeneity was increased year by year. There was a mutation (A --> C) at No. 66 nucleotide of VP1 of EV71 that two strains were isolated in 2003 and 8 strains in 2008, that caused amino acid mutation (Q --> H) at No. 22 of VP1.
EV71 C4b was gradually moved to C4a from 1998 to 2008. There was a missense mutation at No. 66 nucleotide of VP1.
分析深圳肠道病毒71型（EV71）VP1基因的进化情况。
采用EV71特异性引物通过逆转录聚合酶链反应（RT-PCR）对所有样本进行检测。扩增并测序EV71的VP1基因。使用DNAStar、BioEdit和Mega 3.1软件，通过将序列与A、B和C亚基因型进行比较构建系统发育树。
35株病毒中，VP1核苷酸序列的同源性在92.1% - 100%之间。与A和B亚基因型的VP1核苷酸序列同源性在81.4% - 91.1%之间。深圳35株病毒的VP1核苷酸序列与C4簇的同源性在93% - 97.4%之间。1998年至2004年EV71的流行株为C4b亚基因型，自2003年起逐渐向C4a亚基因型转变。2006年至2008年所有EV71均为C4b亚基因型。此外，2003年至2008年与安徽FY23 EV71毒株的VP1核苷酸序列同源性分别为94.5% - 94.7%、95.7% - 95.8%、96.2%、95.4% - 97.5%、96.3% - 99.2%。表明同源性逐年增加。在2003年分离的2株和2008年分离的8株EV71的VP1基因第66位核苷酸发生了A→C突变，导致VP1第22位氨基酸发生Q→H突变。
1998年至2008年EV71 C4b亚基因型逐渐向C4a亚基因型转变。VP1基因第66位核苷酸发生错义突变。
