Animal Physiology Laboratory, Department of Animal Science, University of Ibadan, Ibadan, Oyo State, Nigeria.
Anim Reprod Sci. 2010 Jun;119(3-4):282-6. doi: 10.1016/j.anireprosci.2009.12.001. Epub 2009 Dec 16.
The influence of fumonisin B(1) (a mycotoxin produced by Fusarium verticillioides) on sperm reserves and production of crossbred pubertal rabbits was studied using an experimental model that lasted 28 weeks. Forty-eight male rabbits, 7 weeks old and with average weight of 757.50+/-0.50 g, were allotted to four dietary fumonisin B(1) concentrations of 0.13, 5.0, 7.5 and 10.0 mg kg(-1) constituting diets 1 (control), 2, 3 and 4, respectively. The paired testes weight of rabbits fed diet 3 was significantly (P<0.05) higher than those fed diet 2 and the control. However, the epididymal weight was significantly (P<0.05) lower in rabbits fed the control diet as compared to others on test diets. The gonadal sperm reserves of the animals were significantly (P<0.05) reduced by the toxin with increased concentrations of the toxin in the diets. The sperm reserves per testis and per gram testis were significantly (P<0.05) higher in the control rabbits than those fed diets 3 and 4. The sperm reserves in caput, corpus and caudal epididymis declined significantly with each increase in the fumonisin concentration in the diets. The number of spermatozoa in total caput, corpus and cauda was significantly (P<0.05) higher in rabbits fed the control diet and the least in rabbits fed diet 4 containing 10.0mg fumonisin B(1)/kg. Extra-gonadal sperm reserves significantly decreased (P<0.05) in rabbits fed diets 3 and 4 compared to the control. The daily sperm production of the animals fed diets 2, 3 and 4 declined significantly to 67, 59 and 36% relative to those animals fed the control diet. This study suggests that exposure of breeding male rabbits to diets contaminated with fumonisin B(1) up to 7.5 mg fumonisin B(1)/kg will depress testicular and epididymal sperm reserves and sperm production and potentially impair reproduction in the animals.
本研究采用 28 周的实验模型，探讨了伏马菌素 B1（一种由串珠镰刀菌产生的真菌毒素）对杂交青春期公兔精子储备和产生的影响。48 只 7 周龄、平均体重为 757.50±0.50g 的雄性兔子，被分配到 4 种不同伏马菌素 B1 浓度的日粮中，浓度分别为 0.13、5.0、7.5 和 10.0mg/kg，分别构成日粮 1（对照）、2、3 和 4。与日粮 2 和对照相比，日粮 3 组兔子的配对睾丸重量显著（P<0.05）更高。然而，与其他试验日粮相比，对照组兔子的附睾重量显著（P<0.05）更低。动物的睾丸精子储备随着日粮中毒素浓度的增加而显著（P<0.05）减少。与对照组相比，每只睾丸和每克睾丸的精子储备在对照兔中显著（P<0.05）更高。随着日粮中伏马菌素浓度的增加，睾丸头部、体部和尾部的精子储备显著（P<0.05）减少。总头部、体部和尾部精子总数在对照组兔子中显著（P<0.05）更高，而在日粮 4 组（含 10.0mg 伏马菌素 B1/kg）中最低。与对照组相比，日粮 3 和 4 组兔子的附睾外精子储备显著（P<0.05）减少。与对照组相比，饲喂日粮 2、3 和 4 的动物的日精子产量分别显著（P<0.05）下降了 67%、59%和 36%。本研究表明，雄性繁殖兔暴露于含有 7.5mg 伏马菌素 B1/kg 的日粮中，会抑制睾丸和附睾精子储备和精子产生，并可能损害动物的繁殖能力。
