Animal Physiology Laboratory, Department of Animal Science, University of Ibadan, Ibadan, Nigeria.
J Anim Physiol Anim Nutr (Berl). 2009 Dec;93(6):726-31. doi: 10.1111/j.1439-0396.2008.00862.x.
In a 196-day feeding trial, 48 male crossbred rabbits (New Zealand × Chinchilla) were randomly assigned and fed varied dietary fumonisin levels of 0.13, 5.0, 7.5 and 10.0 mg fumonisin B(1)/kg diet constituting treatments 1 (control), 2, 3 and 4 respectively. Five animals were randomly selected, stunned and killed per treatment. Relative weight of various visceral organs examined except heart and adrenal gland were significantly (p < 0.05) influenced by the dietary treatments. Liver and spleen weights of rabbits fed 10.0 mg fumonisin per kg were significantly (p < 0.05) lower than those fed control diet and diet 2. Kidney and testes weights were significantly (p < 0.05) lower in rabbits fed control diet and increased with increase in the dietary fumonisin levels. Histological examination of the organs revealed that rabbits fed diets 2, 3 and 4 showed increased severe lesion of approximately 20%, 40% and 60%, respectively, of the total slides examined for each treatment. Forty per cent and 80% of the rabbits fed diets containing 7.5 and 10.0 mg/kg fumonisin, respectively, showed severe necrosis whereas 40%, 60% and 20% of the rabbits fed 5.0, 7.5 and 10.0 mg/kg, respectively, showed mild–moderate liver necrosis/lesions as compared with non-significant lesion observed in the controls. Testicles of rabbits fed diets 3 and 4 showed mild–moderate lesions and sertoli cell degeneration. Tunica mucosa erosion was observed and predominant in the stomach and small intestine of rabbits fed 7.5 and 10.0 mg fumonisin per kg diet. This suggested that fumonisin B(1) above 5.0 mg/kg in rabbit diet is toxic to body organs with potential to induce their hypofunction or total damage.
在一项为期 196 天的喂养试验中，48 只雄性杂交兔（新西兰兔×青紫蓝兔）被随机分配并喂食不同水平的玉米赤霉烯酮，剂量分别为 0.13、5.0、7.5 和 10.0 mg 玉米赤霉烯酮 B(1)/kg 日粮，分别构成处理 1（对照）、2、3 和 4。每个处理组随机选择 5 只动物，进行麻醉和处死。除心脏和肾上腺外，检查的各种内脏器官的相对重量均受到日粮处理的显著影响（p<0.05）。喂食 10.0 mg 玉米赤霉烯酮/公斤日粮的兔子的肝脏和脾脏重量明显低于喂食对照日粮和日粮 2 的兔子。喂食对照日粮和玉米赤霉烯酮水平增加的兔子的肾脏和睾丸重量显著降低（p<0.05）。器官的组织学检查表明，喂食日粮 2、3 和 4 的兔子分别表现出约 20%、40%和 60%的总切片严重病变增加。分别喂食含 7.5 和 10.0 mg/kg 玉米赤霉烯酮日粮的 40%和 80%的兔子出现严重坏死，而分别喂食 5.0、7.5 和 10.0 mg/kg 日粮的 40%、60%和 20%的兔子出现轻度至中度肝脏坏死/病变，与对照相比无明显病变。喂食日粮 3 和 4 的兔子的睾丸出现轻度至中度病变和支持细胞退化。在喂食 7.5 和 10.0 mg 玉米赤霉烯酮/公斤日粮的兔子的胃和小肠中观察到黏膜侵蚀，且占主导地位。这表明，日粮中玉米赤霉烯酮 B(1)含量超过 5.0 mg/kg 对兔子的器官有毒性，可能导致其功能减退或全部损伤。
