University of Groningen, University Medical Center Groningen (UMCG), University Center Psychiatry (UCP) Section Biological Psychiatry. P.O. Box 30.001 9700 RB Groningen, The Netherlands.
Trends Biotechnol. 2010 Mar;28(3):150-8. doi: 10.1016/j.tibtech.2009.12.005. Epub 2010 Jan 14.
In medicine and biotechnology, close monitoring of molecular processes might assist to optimise therapeutic interventions and production of biochemicals, respectively. Here, we summarize the current status of two automatic and continuous sampling technologies, microdialysis and microfiltration, which facilitate both in vivo and in vitro monitoring of nearly any analyte, because they can be combined easily with many analytical techniques. Conventional microdialysis and microfiltration, which require collecting relatively large samples, are however often impractical and semi-quantitative; hence, we focus on ultraslow sampling to circumvent such limitations. Ultraslow microdialysis and microfiltration already have been used successfully for quantitative pharmacokinetics, glucose metabolism (e.g. of the brain), cytokines and proteomics (e.g. tumour secretomes), both in vivo and in vitro.
在医学和生物技术领域，对分子过程的密切监测可能有助于优化治疗干预和生物化学制品的生产。在这里，我们总结了两种自动连续采样技术——微透析和微滤的现状，这两种技术都可以方便地与许多分析技术结合，从而实现对几乎任何分析物的体内和体外监测。然而，传统的微透析和微滤需要收集相对较大的样本，因此往往不切实际且只能进行半定量分析；因此，我们专注于超慢速采样来规避这些限制。超慢速微透析和微滤已经成功地用于体内和体外的定量药代动力学、葡萄糖代谢（如大脑）、细胞因子和蛋白质组学（如肿瘤分泌组）。
