Children's National Medical Center, Center for Genetic Medicine Research, 111 Michigan Avenue NW, Washington, DC 20010, USA.
Expert Rev Proteomics. 2012 Jun;9(3):337-45. doi: 10.1586/epr.12.21.
Studies of the cell secretome have greatly increased in recent years owing to improvements in proteomic platforms, mass spectrometry instrumentation and to the increased interaction between analytical chemists, biologists and clinicians. Several secretome studies have been implemented in different areas of research, leading to the generation of a valuable secretome catalogs. Secreted proteins continue to be an important source of biomarkers and therapeutic target discovery and are equally valuable in the field of microbiology. Several discoveries have been achieved in vitro using cell culture systems, ex vivo using human tissue specimens and in vivo using animal models. In this review, some of the most recent advances in secretome studies and the fields that have benefited the most from this evolving technology are highlighted.
近年来，由于蛋白质组学平台、质谱仪器的改进以及分析化学家、生物学家和临床医生之间的互动增加，细胞分泌组的研究有了很大的进展。在不同的研究领域已经进行了几项分泌组研究，产生了有价值的分泌组目录。分泌蛋白仍然是生物标志物和治疗靶点发现的重要来源，在微生物学领域同样具有价值。已经在体外使用细胞培养系统、在体外使用人体组织标本和在体内使用动物模型取得了一些发现。在这篇综述中，强调了分泌组研究的一些最新进展，以及从这项不断发展的技术中受益最多的领域。
