G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Proc Natl Acad Sci U S A. 2010 Jan 26;107(4):1289-94. doi: 10.1073/pnas.0910785107. Epub 2010 Jan 6.
The remarkable deformability of the human red blood cell (RBC) results from the coupled dynamic response of the phospholipid bilayer and the spectrin molecular network. Here we present quantitative connections between spectrin morphology and membrane fluctuations of human RBCs by using dynamic full-field laser interferometry techniques. We present conclusive evidence that the presence of adenosine 5'-triphosphate (ATP) facilitates non-equilibrium dynamic fluctuations in the RBC membrane that are highly correlated with the biconcave shape of RBCs. Spatial analysis of the fluctuations reveals that these non-equilibrium membrane vibrations are enhanced at the scale of spectrin mesh size. Our results indicate that the dynamic remodeling of the coupled membranes powered by ATP results in non-equilibrium membrane fluctuations manifesting from both metabolic and thermal energies and also maintains the biconcave shape of RBCs.
人类红细胞（RBC）的显著变形性源于磷脂双层和血影蛋白分子网络的耦合动态响应。在这里，我们通过使用动态全场激光干涉技术，呈现了血影蛋白形态与人类 RBC 膜波动之间的定量关系。我们提供确凿的证据表明，三磷酸腺苷（ATP）的存在促进了 RBC 膜的非平衡动力学波动，这与 RBC 的双凹形高度相关。波动的空间分析表明，这些非平衡膜振动在血影蛋白网格尺寸的尺度上得到增强。我们的结果表明，由 ATP 驱动的耦合膜的动态重塑导致了非平衡膜波动，这些波动来自代谢和热能，并维持 RBC 的双凹形。
