Department of Genome Sciences, University of Washington, Seattle, Washington, USA.
Nat Methods. 2010 Feb;7(2):119-22. doi: 10.1038/nmeth.1416. Epub 2010 Jan 17.
We demonstrate subassembly, an in vitro library construction method that extends the utility of short-read sequencing platforms to applications requiring long, accurate reads. A long DNA fragment library is converted to a population of nested sublibraries, and a tag sequence directs grouping of short reads derived from the same long fragment, enabling localized assembly of long fragment sequences. Subassembly may facilitate accurate de novo genome assembly and metagenome sequencing.
我们展示了亚组装(subassembly)，这是一种体外文库构建方法，它扩展了短读测序平台在需要长、准确读长的应用中的用途。长 DNA 片段文库被转化为嵌套亚文库的群体，标签序列指导来自同一长片段的短读的分组，从而实现长片段序列的本地化组装。亚组装可以促进准确的从头基因组组装和宏基因组测序。
