黑曲霉在不同生长条件下利用固态发酵生产脂肪酶。

Lipase production by Aspergillus niger under various growth conditions using solid state fermentation.

作者信息

Olama Z A, el-Sabaeny A H

机构信息

Microbiology and Botany Department, Faculty of Science Alexandria University, Egypt.

出版信息

Microbiologia. 1993 Dec;9(2):134-41.

PMID:8172691

Abstract

Ricinus seed litters were chosen as a cheap carbon source for lipase production by A. niger under solid state fermentation (SSF). Maximum lipase production was achieved upon using an enriched (potassium citrate and casein) waste at pH 7.8 and 30 degrees C for 8 days incubation. Nitrogen sources as NH4Cl, NH4NO3, (NH4)2SO4, urea and amino acids repressed the lipolytic activity. The chloride salts of Ba2+, Co2+, Cu2+, Fe3+, Hg2+, K+, Mg2+, Mn2+, Na+ and Sn2+ inhibited, while Zn+2 did not affect lipase production. Compounds containing hydrolyzable ester group, such as Tween(s), were found to inhibit lipase activity. When the effect of different additives such as EDTA, gum acacia, span(s), mineral and vitamins, were studied, it was found that they all exhibit decreased lipase production by the tested fungus.

摘要

蓖麻籽废弃物被选为黑曲霉在固态发酵（SSF）条件下生产脂肪酶的廉价碳源。在pH 7.8、30℃条件下，使用富含（柠檬酸钾和酪蛋白）的废弃物培养8天，可实现脂肪酶的最大产量。氯化铵、硝酸铵、硫酸铵、尿素和氨基酸等氮源会抑制脂解活性。Ba2+、Co2+、Cu2+、Fe3+、Hg2+、K+、Mg2+、Mn2+、Na+和Sn2+的氯化物盐会抑制脂肪酶的产生，而Zn2+则不影响脂肪酶的产生。发现含有可水解酯基的化合物，如吐温，会抑制脂肪酶活性。当研究不同添加剂如乙二胺四乙酸（EDTA）、阿拉伯胶、司盘、矿物质和维生素的作用时，发现它们都会使受试真菌的脂肪酶产量降低。