Department of Biotechnology, Central Leather Research Institute, Adyar, Chennai 600 020, India.
J Agric Food Chem. 2009 Nov 25;57(22):10658-63. doi: 10.1021/jf902726p.
Corn steep liquor (CSL) has been used as a nutrition adjunct for the production of an extracellular lipase from Aspergillus niger, which has immense importance as an additive in laundry detergent formulations. A five-level four-factorial central composite design was chosen to determine the optimal medium components with four critical variables, namely, CSL, NH4H2PO4, Na2HPO4, and sesame oil, that were found to be influential for lipase production by the classical one-factor-at-a-time method. The model suggested that all of the factors chosen had a significant impact on lipase production, and the optimum values of the influential parameters were CSL, 2.0%, w/v; NH4H2PO4, 0.05%, w/v; Na2HPO4, 0.75%, w/v; and sesame oil, 2.0%, w/v, with an activity of 26.7 U/mL at 48 h and 30 degrees C, which was 2.16-fold higher than the initial activity (12 U/mL) obtained by the conventional one-factor-at-a-time method. Furthermore, the enzyme has good potential for the hydrolysis of vegetable oils and fish oils, and a hydrolytic ratio of 88.73% was obtained with palm oil at 48 h. The utilization of CSL and sesame oil for lipase production from A. niger makes the process green, because both are renewable substrates and economically viable at an industrial scale.
玉米浆（CSL）已被用作生产黑曲霉胞外脂肪酶的营养添加剂，作为洗衣洗涤剂配方中的添加剂具有重要意义。选择了五水平四因素中心组合设计，以确定具有四个关键变量的最佳培养基成分，这四个关键变量是玉米浆（CSL）、NH4H2PO4、Na2HPO4 和芝麻油，它们通过经典的单因素逐一法被发现对脂肪酶生产有影响。该模型表明，所选择的所有因素都对脂肪酶生产有显著影响，有影响的参数的最佳值为 CSL，2.0%，w/v；NH4H2PO4，0.05%，w/v；Na2HPO4，0.75%，w/v；芝麻油，2.0%，w/v，在 48 小时和 30 摄氏度下的酶活为 26.7 U/mL，比传统单因素逐一法获得的初始酶活（12 U/mL）高 2.16 倍。此外，该酶对植物油和鱼油具有良好的水解潜力，在 48 小时内用棕榈油获得了 88.73%的水解比。黑曲霉生产脂肪酶时利用玉米浆和芝麻油使工艺绿色化，因为两者都是可再生的底物，在工业规模上具有经济可行性。
