人细小病毒 B19 感染与冠状动脉粥样硬化。

Human parvovirus b19 infection in patients with coronary atherosclerosis.

机构信息

Min-Sheng Hospital, Tao-Yuan, Taiwan.

出版信息

Arch Med Res. 2009 Oct;40(7):612-7. doi: 10.1016/j.arcmed.2009.09.002.

DOI:10.1016/j.arcmed.2009.09.002

PMID:20082878

Abstract

BACKGROUND AND AIMS

The identification of possible pathogens for an infectious etiology of atherosclerotic coronary artery disease (CAD) is an expanding field. The present study was undertaken to explore the role of parvovirus B19, a potent infectious agent.

METHODS

A total of 565 patients were analyzed (90 patients with CAD, and 475 controls). Serologic analysis for human paravovirus B19 (B19) specific IgM and IgG was carried out in all patients. In addition, tissue specimens were obtained from five patients who received heart transplants. Direct in situ polymerase chain reaction (PCR) and immunocytochemistry were performed in the samples to localize B19 DNA.

RESULTS

Enzyme immunoassay showed that the seropositive rate of anti-B19 IgG in patients with CAD was 1.5- to 2.7-fold more frequent than in healthy controls. Clinical characteristics did not affect the prevalence of seropositivity for B19. However, anti-B19 IgM and B19-specific DNA were not detected in healthy or individuals with CAD. Furthermore, nonradioactive in situ PCR found that the majority of B19-specific DNA was located in the endothelial cells of the thickened intima.

CONCLUSIONS

Our results first demonstrate a seroepidemiological and histopathological association between chronic B19 infection and CAD, suggesting that B19 infection may have a potential role in the pathogenesis of coronary atherosclerosis.

摘要

背景与目的

确定动脉粥样硬化性冠心病（CAD）感染病因的可能病原体是一个不断发展的领域。本研究旨在探讨细小病毒 B19 的作用，这是一种强有力的传染性病原体。

方法

分析了 565 例患者（90 例 CAD 患者和 475 例对照）。对所有患者进行了人细小病毒 B19（B19）特异性 IgM 和 IgG 的血清学分析。此外，从接受心脏移植的 5 例患者中获得组织标本。对样品进行直接原位聚合酶链反应（PCR）和免疫细胞化学检测，以定位 B19 DNA。

结果

酶免疫测定显示，CAD 患者抗 B19 IgG 的血清阳性率比健康对照组高 1.5-2.7 倍。临床特征并不影响 B19 血清阳性率。然而，在健康个体或 CAD 患者中未检测到抗 B19 IgM 和 B19 特异性 DNA。此外，非放射性原位 PCR 发现，大多数 B19 特异性 DNA 位于增厚内膜的内皮细胞中。