经香叶醇处理的神经胶质瘤细胞（A172）的动态蛋白质组学概述。
Dynamic proteomic overview of glioblastoma cells (A172) exposed to perillyl alcohol.
机构信息
Laboratory for Protein Chemistry, Chemistry Institute, and the Rio de Janeiro Proteomic Network, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
出版信息
J Proteomics. 2010 Mar 10;73(5):1018-27. doi: 10.1016/j.jprot.2010.01.003. Epub 2010 Jan 18.
Abstract
Perillyl alcohol (POH) is a naturally occurring terpene and a promising chemotherapeutic agent for glioblastoma multiform; yet, little is known about its molecular effects. Here we present results of a semi-quantitative proteomic analysis of A172 cells exposed to POH for different time-periods (1', 10', 30', 60', 4h, and 24h). The analysis identified more than 4000 proteins; which were clustered using PatternLab for proteomics and then linked to Ras signaling, tissue homeostasis, induction of apoptosis, metallopeptidase activity, and ubiquitin-protein ligase activity. Our results make available one of the most complete protein repositories for the A172. Moreover, we detected the phosphorylation of GSK3beta (Glycogen synthase kinase) and the inhibition of ERK's (extracellular signal regulated kinase) phosphorylation after 10', which suggests a new mechanism of POH's activation for apoptosis.
摘要
芳樟醇（POH）是一种天然存在的萜烯，也是多形性胶质母细胞瘤有前途的化疗药物；然而，关于其分子作用知之甚少。在这里，我们展示了对 A172 细胞暴露于 POH 不同时间段（1'、10'、30'、60'、4h 和 24h）的半定量蛋白质组学分析的结果。该分析鉴定了超过 4000 种蛋白质；PatternLab for proteomics 使用这些蛋白质进行聚类，然后与 Ras 信号、组织稳态、细胞凋亡诱导、金属肽酶活性和泛素-蛋白连接酶活性相关联。我们的结果为 A172 提供了其中一个最完整的蛋白质存储库。此外，我们在 10'后检测到 GSK3beta（糖原合酶激酶）的磷酸化和 ERK 的磷酸化抑制，这表明 POH 激活细胞凋亡的新机制。
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