分析差异 shotgun 蛋白质组学中的边缘案例。
Analyzing marginal cases in differential shotgun proteomics.
机构信息
Center for Technological Development in Health, Oswaldo Cruz Institute, Rio de Janeiro, Brazil.
出版信息
Bioinformatics. 2011 Jan 15;27(2):275-6. doi: 10.1093/bioinformatics/btq632. Epub 2010 Nov 11.
Abstract
SUMMARY
We present an approach to statistically pinpoint differentially expressed proteins that have quantitation values near the quantitation threshold and are not identified in all replicates (marginal cases). Our method uses a Bayesian strategy to combine parametric statistics with an empirical distribution built from the reproducibility quality of the technical replicates.
AVAILABILITY
The software is freely available for academic use at http://pcarvalho.com/patternlab.
摘要
摘要
我们提出了一种方法，用于统计定位定量值接近定量阈值且在所有重复（边缘情况）中均未鉴定出的差异表达蛋白。我们的方法使用贝叶斯策略将参数统计与从技术重复的重现性质量构建的经验分布相结合。
可及性
该软件可免费在学术上使用，网址为 http://pcarvalho.com/patternlab。
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