Kozak Igor, Cheng Lingyub, Rought Steffney, Woelk Christopher, Hardiman Gary, Barron Erin, Schrier Rachel D, Corbeil Jacques, Freeman William R
Jacobs Retina Center, Shiley Eye Center, Department of Ophthalmology, University of California, San Diego, La Jolla, California 92093, USA.
Retina. 2010 Jun;30(6):952-7. doi: 10.1097/IAE.0b013e3181c700f8.
The purpose of this study was to determine the cytokine-related pathogenesis of human immunodeficiency virus retinopathy in human autopsy eyes.
Fresh autopsy eyes were procured from clinically diagnosed patients with acquired immunodeficiency syndrome who had died as a result of disease-related complications; eyes were immediately immersed in RNAlater. Clean 2-mm trephines were used to punch individual pathologic retina in areas of cotton-wool spots and control punches. Total RNA was extracted using the TRIzol extraction protocol, and the optimal density of the RNA was measured at an optical density of 260 nm. [Delta]Ct (cytokine) values were calculated using the comparative cytokine analysis method. The results are expressed as a mean fold modulation and as a statistical comparison of Ct values controlling for retinal areas without a lesion in the same eye.
The fold modulations and the statistical comparisons of the cytokines studied in tissues from cotton-wool spots and control retina, respectively, regulated on activation normal T cell expressed and secreted (RANTES), macrophage inflammatory protein 1beta, macrophage inflammatory protein 1alpha (5.32x, P = 0.04), and Bcl-2-associated X protein (1.24x, P = 0.05) had a marked elevation of fold modulation and were statistically significant compared with control tissue. Interleukin-8 (1.09x, P = 0.18), interleukin-4, and interleukin-10 (2.7x, P = 0.30) were not significantly expressed in cotton-wool spots.
Certain inflammatory human immunodeficiency virus-associated and apoptotic cytokines are expressed in cotton-wool spots in eyes with human immunodeficiency virus retinopathy.
本研究旨在确定人类免疫缺陷病毒视网膜病变在人类尸检眼中细胞因子相关的发病机制。
从临床诊断为获得性免疫缺陷综合征且因疾病相关并发症死亡的患者获取新鲜尸检眼；将眼睛立即浸入RNA保护剂中。使用干净的2毫米环钻在棉绒斑区域和对照打孔处冲压单个病理视网膜。使用TRIzol提取方案提取总RNA，并在260nm光密度下测量RNA的最佳密度。使用比较细胞因子分析方法计算ΔCt（细胞因子）值。结果以平均倍数调节表示，并作为对同一只眼中无病变视网膜区域的Ct值的统计比较。
分别在棉绒斑组织和对照视网膜中研究的细胞因子的倍数调节和统计比较中，调节激活正常T细胞表达和分泌（RANTES）、巨噬细胞炎性蛋白1β、巨噬细胞炎性蛋白1α（5.32倍，P = 0.04）和Bcl-2相关X蛋白（1.24倍，P = 0.05）的倍数调节有明显升高，与对照组织相比具有统计学意义。白细胞介素-8（1.09倍，P = 0.18）、白细胞介素-4和白细胞介素-10（2.7倍，P = 0.30）在棉绒斑中未显著表达。
某些与人类免疫缺陷病毒相关的炎性细胞因子和凋亡细胞因子在患有人类免疫缺陷病毒视网膜病变的眼睛的棉绒斑中表达。
