Tampere University of Technology, Department of Biomedical Engineering, PO Box 692, 33101 Tampere, Finland.
J Tissue Eng Regen Med. 2010 Jul;4(5):366-73. doi: 10.1002/term.249.
Porous polylactide/beta-tricalcium phosphate (PLA/beta-TCP) composite scaffolds were fabricated by freeze-drying. The aim of this study was to characterize these graded porous composite scaffolds in two different PLA concentrations (2 and 3 wt%). Also, three different beta-TCP ratios (5, 10 and 20 wt%) were used to study the effect of beta-TCP on the properties of the polymer. The characterization was carried out by determining the pH, weight change, component ratios, thermal stability, inherent viscosity and microstructure of the scaffolds in 26 weeks of hydrolysis. This study indicated that no considerable change was noticed in the structure of the scaffolds when the beta-TCP filler was added. Also, the amount of beta-TCP did not affect the pore size or the pore distribution in the scaffolds. We observed that the fabrication method improved the thermal stability of the samples. Our results suggest that, from the structural point of view, these scaffolds could have potential for the treatment of osteochondral defects in tissue engineering applications. The porous bottom surface of the scaffold and the increased osteogenic differentiation potential achieved with beta-TCP particles may encourage the growth of bone cells. In addition, the dense surface skin of the scaffold may inhibit the ingrowth of osteoblasts and bone tissue, while simultaneously encouraging the ingrowth of chondrocytes.
多孔聚乳酸/β-磷酸三钙（PLA/β-TCP）复合支架通过冷冻干燥法制备。本研究的目的是在两种不同 PLA 浓度（2 和 3wt%）下对这些分级多孔复合支架进行表征。此外，还使用了三种不同的β-TCP 比例（5、10 和 20wt%）来研究β-TCP 对聚合物性能的影响。通过在水解的 26 周内测定支架的 pH 值、重量变化、组分比、热稳定性、固有粘度和微观结构来进行表征。这项研究表明，当添加β-TCP 填充剂时，支架的结构没有明显变化。此外，β-TCP 的含量不会影响支架中的孔径或孔分布。我们观察到，该制造方法提高了样品的热稳定性。我们的结果表明，从结构角度来看，这些支架可能在组织工程应用中具有治疗骨软骨缺陷的潜力。支架的多孔底面和β-TCP 颗粒实现的增加的成骨分化潜力可能会促进骨细胞的生长。此外，支架的致密表面皮肤可能会抑制成骨细胞和骨组织的向内生长，同时鼓励软骨细胞的向内生长。
