Park Hyunchul, Ryu Jaeyoung, Jung Seunggon, Park Hongju, Oh Heekyun, Kook Minsuk
Bitgoeul Dental Clinic 2F, Doklibro 8 Nam-gu, Gwangju 61660, Korea.
Department of Oral and Maxillofacial Surgery, School of Dentistry, Chonnam National University, Gwangju 61186, Korea.
Materials (Basel). 2022 Jan 21;15(3):827. doi: 10.3390/ma15030827.
The need for the repair of bone defects has been increasing due to various causes of loss of skeletal tissue. High density polyethylenes (HDPE) have been used as bone substitutes due to their excellent biocompatibility and mechanical strength. In the present study, we investigated the preosteoblast cell proliferation and differentiation on the adding nano-hydroxyapatite (n-HAp) particles into HDPE scaffold and treating HDPE/n-HAp scaffolds with nitrogen (N) plasma. The three-dimensional (3D) HDPE/n-HAp scaffolds were prepared by fused modeling deposition 3D printer. The HDPE/n-HAp was blended with 10 wt% of n-HAp particle. The scaffold surface was reactive ion etched with nitrogen plasma to improve the preosteoblast biological response in vitro. After N plasma treatment, surfaces characterizations were investigated using Fourier transform infrared spectroscopy, scanning electron microscopy, and atomic force microscopy. The proliferation and differentiation of preosteoblast (MC3T3-E1) cells were evaluated by MTT assay and alkaline phosphatase (ALP) activity. The incorporation of n-HAp particles and N plasma surface treatment showed the improvement of biological responses of MC3T3-E1 cells in the HDPE scaffolds.
由于骨骼组织丧失的各种原因，骨缺损修复的需求一直在增加。高密度聚乙烯（HDPE）因其优异的生物相容性和机械强度而被用作骨替代物。在本研究中，我们研究了在HDPE支架中添加纳米羟基磷灰石（n-HAp）颗粒并用氮（N）等离子体处理HDPE/n-HAp支架对前成骨细胞增殖和分化的影响。三维（3D）HDPE/n-HAp支架由熔融建模沉积3D打印机制备。HDPE/n-HAp与10 wt%的n-HAp颗粒混合。用氮等离子体对支架表面进行反应离子蚀刻，以改善体外前成骨细胞的生物学反应。N等离子体处理后，使用傅里叶变换红外光谱、扫描电子显微镜和原子力显微镜对表面特性进行了研究。通过MTT法和碱性磷酸酶（ALP）活性评估前成骨细胞（MC3T3-E1）的增殖和分化。n-HAp颗粒的掺入和N等离子体表面处理显示出HDPE支架中MC3T3-E1细胞生物学反应的改善。
