鉴定铜绿假单胞菌作用于支气管上皮细胞时双氧化酶产生过氧化氢的特性。
Characterization of hydrogen peroxide production by Duox in bronchial epithelial cells exposed to Pseudomonas aeruginosa.
机构信息
National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda 20852, USA.
出版信息
FEBS Lett. 2010 Mar 5;584(5):917-22. doi: 10.1016/j.febslet.2010.01.025. Epub 2010 Jan 19.
Abstract
Hydrogen peroxide production by the NADPH oxidase Duox1 occurs during activation of respiratory epithelial cells stimulated by purified bacterial ligands, such as lipopolysaccharide. Here, we characterize Duox activation using intact bacterial cells of several airway pathogens. We found that only Pseudomonas aeruginosa, not Burkholderia cepacia or Staphylococcus aureus, triggers H2O2 production in bronchial epithelial cells in a calcium-dependent but predominantly ATP-independent manner. Moreover, by comparing mutant Pseudomonas strains, we identify several virulence factors that participate in Duox activation, including the type-three secretion system. These data provide insight on Duox activation by mechanisms unique to P. aeruginosa.
摘要
NADPH 氧化酶 Duox1 在呼吸上皮细胞被纯化的细菌配体（如脂多糖）刺激激活时会产生过氧化氢。在这里，我们使用几种气道病原体的完整细菌细胞来表征 Duox 的激活。我们发现，只有铜绿假单胞菌，而不是伯克霍尔德菌或金黄色葡萄球菌，以一种依赖于钙但主要不依赖于 ATP 的方式触发支气管上皮细胞产生 H2O2。此外，通过比较突变的铜绿假单胞菌菌株，我们确定了参与 Duox 激活的几种毒力因子，包括 III 型分泌系统。这些数据为了解铜绿假单胞菌特有的 Duox 激活机制提供了线索。
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