Department of Biohybrid Systems, Fraunhofer Institute for Biomedical Engineering, Ensheimer Str. 48, 66386 St. Ingbert, Germany.
J Biotechnol. 2010 Jul 1;148(1):83-90. doi: 10.1016/j.jbiotec.2010.01.007. Epub 2010 Jan 18.
Human mesenchymal stem cells are promising candidates for cell-based therapies since they have the capacity to differentiate into a variety of cell types. However, the acceptance of hMSCs for clinical applications as well as in vitro tissue models will depend on strategies for standard characterisations. Impedance spectroscopy is a proven and powerful tool for non-invasive monitoring of cellular processes. The aim of this study was to prove the hypothesis, that the process of osteogenic differentiation can be monitored non-invasively and time-continuously by using impedance spectroscopy. This hypothesis was examined for 2D cell layers of hMSCs by continuous impedance spectroscopy employing a planar electrode-based chip and for 3D aggregates of hMSCs after 21 and 25 days of osteogenic treatment by using a capillary measurement system. The impedance spectra of osteogenic treated hMSCs reported a significant increase of the magnitude of impedance compared to controls cultivated in normal growth medium. The osteogenic status of the cells was determined by alkaline phosphatase expression and von Kossa staining. In respect to that finding it is concluded that impedance spectroscopy is an appropriate method for non-invasive characterisation of osteogenic differentiation of hMSCs, which is relevant for quality control of cell-based implants and cell-based test systems for drug development.
人骨髓间充质干细胞是细胞治疗的有前途的候选者，因为它们有能力分化为多种细胞类型。然而，hMSCs 被接受用于临床应用以及体外组织模型，将取决于标准特性的策略。阻抗谱是一种经过验证的强大工具，可用于非侵入性监测细胞过程。本研究的目的是证明这样一个假设，即通过使用阻抗谱可以非侵入性和连续地监测成骨分化过程。通过使用基于平面电极的芯片对 hMSCs 的 2D 细胞层进行连续阻抗谱测量，并在经过 21 和 25 天成骨处理后使用毛细管测量系统对 hMSCs 的 3D 聚集体进行测量，对该假设进行了检验。与在正常生长培养基中培养的对照相比，成骨处理后的 hMSCs 的阻抗谱报告说阻抗的幅度显着增加。细胞的成骨状态通过碱性磷酸酶表达和 von Kossa 染色来确定。根据这一发现，可以得出结论，阻抗谱是 hMSCs 成骨分化的非侵入性特征的合适方法，这对于基于细胞的植入物的质量控制以及药物开发的基于细胞的测试系统是相关的。
