Bounik Raziyeh, Cardes Fernando, Ulusan Hasan, Modena Mario M, Hierlemann Andreas
ETH Zürich, Department of Biosystems Science and Engineering, Basel, Switzerland.
BME Front. 2022 Jun 9;2022:1-21. doi: 10.34133/2022/9857485.
Due to their label-free and noninvasive nature, impedance measurements have attracted increasing interest in biological research. Advances in microfabrication and integrated-circuit technology have opened a route to using large-scale microelectrode arrays for real-time, high-spatiotemporal-resolution impedance measurements of biological samples. In this review, we discuss different methods and applications of measuring impedance for cell and tissue analysis with a focus on impedance imaging with microelectrode arrays in applications. We first introduce how electrode configurations and the frequency range of the impedance analysis determine the information that can be extracted. We then delve into relevant circuit topologies that can be used to implement impedance measurements and their characteristic features, such as resolution and data-acquisition time. Afterwards, we detail design considerations for the implementation of new impedance-imaging devices. We conclude by discussing future fields of application of impedance imaging in biomedical research, in particular applications where optical imaging is not possible, such as monitoring of tissue slices or microelectrode-based brain implants.
由于其无标记和非侵入性的特性，阻抗测量在生物学研究中引起了越来越多的关注。微纳制造和集成电路技术的进步为使用大规模微电极阵列进行生物样品的实时、高时空分辨率阻抗测量开辟了一条途径。在本综述中，我们讨论了用于细胞和组织分析的阻抗测量的不同方法和应用，重点是微电极阵列在应用中的阻抗成像。我们首先介绍电极配置和阻抗分析的频率范围如何决定可提取的信息。然后，我们深入探讨可用于实现阻抗测量的相关电路拓扑及其特征，如分辨率和数据采集时间。之后，我们详细阐述了新型阻抗成像设备实现的设计考虑因素。我们通过讨论阻抗成像在生物医学研究中的未来应用领域来结束本文，特别是在光学成像不可能的应用中，如组织切片监测或基于微电极的脑植入物。
