Department of Obstetrics and Gynaecology, Maastricht University Medical Centre, Center for Reproductive Medicine, Maastricht, The Netherlands.
Hum Reprod. 2010 Mar;25(3):605-12. doi: 10.1093/humrep/dep456. Epub 2010 Jan 18.
In animal models, in vitro culture of preimplantation embryos has been shown to be a risk factor for abnormal fetal outcome, including high and low birthweight. In the human, mean birthweight of singletons after in vitro fertilization (IVF) is considerably lower than after natural conception, but it is not known whether culture conditions play a role in this.
We compared pregnancy rates and perinatal outcomes from singleton pregnancies resulting from a total of 826 first IVF treatment cycles in which oocytes and embryos were randomly allocated to culture in either of two commercially available sequential media systems.
When the 110 live born singletons in the Vitrolife group were compared with the 78 singletons in the Cook group, birthweight +/- SEM (3453 +/- 53 versus 3208 +/- 61 g, P = 0.003), and birthweight adjusted for gestational age and gender (mean z-score +/- SEM: 0.13 +/- 0.09 versus -0.31 +/- 0.10, P = 0.001) were both significantly higher in the Vitrolife group. When analyzed by multiple linear regression together with several other variables that could possibly affect birthweight as covariates, the type of culture medium was significantly (P = 0.01) associated with birthweight.
In vitro culture of human embryos can affect birthweight of live born singletons.
在动物模型中，体外培养胚胎已被证明是导致胎儿异常结局的一个危险因素，包括出生体重过高和过低。在人类中，体外受精（IVF）后单胎的平均出生体重明显低于自然受孕，但尚不清楚培养条件是否在此过程中起作用。
我们比较了总共 826 个首次 IVF 治疗周期中单胎妊娠的妊娠率和围产儿结局，这些周期中卵子和胚胎被随机分配到两种市售的连续培养基系统中进行培养。
当 Vitrolife 组的 110 例活产单胎与 Cook 组的 78 例单胎进行比较时，出生体重 +/- SEM（3453 +/- 53 与 3208 +/- 61 g，P = 0.003），以及按胎龄和性别校正的出生体重（平均 z 评分 +/- SEM：0.13 +/- 0.09 与 -0.31 +/- 0.10，P = 0.001）在 Vitrolife 组中均显著更高。当与其他可能影响出生体重的几个变量一起进行多元线性回归分析作为协变量时，培养基类型与出生体重显著相关（P = 0.01）。
人类胚胎的体外培养可能会影响活产单胎的出生体重。
