Eppley Cancer Institute, Room ECI 3018, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198-6805, USA.
Antimicrob Agents Chemother. 2010 Mar;54(3):1343-6. doi: 10.1128/AAC.01448-09. Epub 2010 Jan 19.
To identify novel anti-HIV-1 peptides based on the antimicrobial peptide database (APD; http://aps.unmc.edu/AP/main.php), we have screened 30 candidates and found 11 peptides with 50% effective concentrations (EC(50)) of <10 microM and therapeutic indices (TI) of up to 17. Furthermore, among the eight peptides (with identical amino acid compositions but different sequences) generated by shuffling the sequence of an aurein 1.2 analog, two had a TI twice that of the original sequence. Because antiviral peptides in the database have an arginine/lysine (R/K) ratio of >1, increases in the Arg contents of amphibian maximin H5 and dermaseptin S9 peptides and the database-derived GLK-19 peptide improved the TIs. These examples demonstrate that the APD is a rich resource and a useful tool for developing novel HIV-1-inhibitory peptides.
为了从抗菌肽数据库 (APD; http://aps.unmc.edu/AP/main.php) 中鉴定新的抗 HIV-1 肽，我们筛选了 30 个候选肽，发现了 11 个 EC(50)值<10 microM 和治疗指数 (TI)高达 17 的肽。此外，在通过改组 aurein 1.2 类似物的序列生成的 8 个肽（具有相同的氨基酸组成但序列不同）中，有两个的 TI 是原始序列的两倍。由于数据库中的抗病毒肽具有精氨酸/赖氨酸 (R/K) 比>1，因此增加了两栖类 maximin H5 和 dermaseptin S9 肽的 Arg 含量以及数据库衍生的 GLK-19 肽提高了 TI。这些例子表明 APD 是开发新型 HIV-1 抑制肽的丰富资源和有用工具。
