Department of Biochemistry, School of Medicine, University of Nairobi P.O. Box 30197 Nairobi, Kenya.
Department of Molecular Biology and Biotechnology, College of Natural and Applied Sciences, Uvumbuzi road, University of Dar es Salaam P.O. Box 35179, Dar es Salaam, Tanzania.
Int J Mol Sci. 2009 Nov 6;10(11):4805-4815. doi: 10.3390/ijms10114805.
Sisal leaf decortications residue (SLDR) is amongst the most abundant agro-industrial residues in Tanzania and is a good feedstock for biogas production. Pre-treatment of the residue prior to its anaerobic digestion (AD) was investigated using a two-stage pre-treatment approach with two fungal strains, CCHT-1 and Trichoderma reesei in succession in anaerobic batch bioreactors. AD of the pre-treated residue with CCTH-1 at 10% (wet weight inoculum/SLDR) inoculum concentration incubated for four days followed by incubation for eight days with 25% (wet weight inoculum/SLDR) of T. reesei gave a methane yield of 0.292 +/- 0.04 m(3) CH(4)/kg volatile solids (VS)(added). On reversing the pre-treatment succession of the fungal inocula using the same parameters followed by AD, methane yield decreased by about 55%. Generally, an increment in the range of 30-101% in methane yield in comparison to the un-treated SLDR was obtained. The results confirmed the potential of CCHT-1 followed by Trichoderma reesei fungi pre-treatment prior to AD to achieve significant improvement in biogas production from SLDR.
剑麻叶去皮残渣（SLDR）是坦桑尼亚最丰富的农业工业残渣之一，是沼气生产的良好原料。使用两步预处理方法，在厌氧分批生物反应器中连续使用两种真菌菌株 CCHT-1 和里氏木霉，对残渣进行预处理，然后进行厌氧消化（AD）。用 CCTH-1 在 10％（湿重接种物/SLDR）接种物浓度下预处理残渣，孵育 4 天，然后用 25％（湿重接种物/SLDR）里氏木霉孵育 8 天，得到 0.292 +/- 0.04 m(3) CH(4)/kg 挥发性固体（VS）（添加）的甲烷产量。用相同的参数反转真菌接种物的预处理顺序，然后进行 AD，甲烷产量下降了约 55％。通常，与未经处理的 SLDR 相比，甲烷产量增加了 30-101％。结果证实，在 AD 之前，使用 CCHT-1 继之以里氏木霉真菌预处理，可显著提高 SLDR 沼气产量。
