Research & Development Center, Hitachi Medical Corporation, Kashiwa, Japan.
Ann Biomed Eng. 2010 Apr;38(4):1664-71. doi: 10.1007/s10439-010-9910-z. Epub 2010 Jan 20.
The effect of scaffold shape on dentin regeneration is not well understood. In this study, porous hydroxyapatite/beta-tricalcium phosphate (HAp/beta-TCP), powdered HAp/beta-TCP, and polyglycolic acid (PGA) fiber mesh were used as scaffolds and transplanted with cultured porcine dental pulp-derived cells into the backs of nude mice. Samples were harvested after 6 weeks. Newly-formed hard tissue was observed in all transplants. When porous HAp/beta-TCP was used, dentin-like hard tissue was observed on the inner wall with minimum cell inclusions and odontoblast-like cells were aligned adjacent to the hard tissue. When HAp/beta-TCP powders or PGA were used, bone-like hard tissues showed cell inclusions and cell alignment was not observed. Hard tissue from the HAp/beta-TCP block group was positive for type I collagen, osteonectin, bone sialoprotein and dentin sialoprotein (DSP), which are markers for dentin. This result was confirmed by in situ hybridization with a dsp probe. Only the aligned cells were positive with an antisense probe. On the other hand, hard tissue from other scaffolds showed incomplete expression of both bone and dentin markers and they were negative for osteonectin and DSP. These results suggest that scaffold shape affects the type of tissue regenerated by dental pulp-derived cells.
支架形状对牙本质再生的影响尚不清楚。本研究中，多孔羟基磷灰石/β-磷酸三钙（HAp/β-TCP）、粉末状 HAp/β-TCP 和聚乙二醇酸（PGA）纤维网被用作支架，并将培养的猪牙髓来源细胞移植到裸鼠背部。6 周后采集样本。所有移植部位均观察到新形成的硬组织。使用多孔 HAp/β-TCP 时，在内壁观察到类似于牙本质的硬组织，细胞内含物最少，牙本质样细胞排列在硬组织旁边。使用 HAp/β-TCP 粉末或 PGA 时，观察到类似于骨的硬组织，其中含有细胞内含物，且未观察到细胞排列。HAp/β-TCP 块组的硬组织对Ⅰ型胶原、骨桥蛋白、骨唾液蛋白和牙本质唾液蛋白（DSP）呈阳性，这些标志物是牙本质的标志。这一结果通过使用 dsp 探针的原位杂交得到了证实。只有排列整齐的细胞对反义探针呈阳性。另一方面，其他支架的硬组织对骨和牙本质标志物的表达均不完整，且对骨桥蛋白和 DSP 呈阴性。这些结果表明，支架形状影响牙髓来源细胞再生的组织类型。
