Research and Development Center, Hitachi Medical Corporation, Kashiwa, Japan.
J Biomed Mater Res A. 2010 Sep 1;94(3):800-5. doi: 10.1002/jbm.a.32749.
Recently, the possibility of tooth tissue engineering has been reported. Although there are a number of available materials, information about scaffolds for tooth tissue engineering is still limited. To improve the manageability of tooth tissue engineering, the effect of scaffolds on in vivo tooth regeneration was evaluated. Collagen and fibrin were selected for this study based on the biocompatibility to dental papilla-derived cells and the results were compared with those of polyglycolic acid (PGA) fiber and beta-tricalcium phosphate (beta-TCP) porous block, which are commonly used for tooth, dentin and bone tissue engineering. Isolated porcine tooth germ-derived cells were seeded onto one of those scaffolds and transplanted to the back of nude mice. Tooth bud-like structures were observed more frequently in collagen and fibrin gels than on PGA or beta-TCP, while the amount of hard tissue formation was less. The results showed that collagen and fibrin gel support the initial regeneration process of tooth buds possibly due to their ability to support the growth of epithelial and mesenchymal cells. On the other hand, maturation of tooth buds was difficult in fibrin and collagen gels, which may require other factors.
最近，有报道称牙齿组织工程具有可能性。尽管有许多可用的材料，但关于牙齿组织工程支架的信息仍然有限。为了提高牙齿组织工程的可操作性，评估了支架对体内牙齿再生的影响。本研究选择了胶原蛋白和纤维蛋白，基于其对牙髓衍生细胞的生物相容性，并将结果与聚乙二醇酸（PGA）纤维和β-磷酸三钙（β-TCP）多孔块进行了比较，后者常用于牙齿、牙本质和骨组织工程。将分离的猪牙胚来源的细胞接种到这些支架之一上，并移植到裸鼠的背部。在胶原蛋白和纤维蛋白凝胶中观察到牙蕾样结构的频率高于 PGA 或β-TCP，而硬组织形成的量较少。结果表明，胶原蛋白和纤维蛋白凝胶可能由于其支持上皮和间充质细胞生长的能力，支持牙蕾的初始再生过程。另一方面，牙蕾在纤维蛋白和胶原蛋白凝胶中的成熟较为困难，这可能需要其他因素。
