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狂犬病抗原检测快速方法的验证及实际应用

Validation and operational application of a rapid method for rabies antigen detection.

作者信息

Saturday Greg A, King Robin, Fuhrmann Leslie

机构信息

Veterinary Laboratory Europe, Landstuhl, Germany.

出版信息

US Army Med Dep J. 2009 Jan-Mar:42-5.

PMID:20088045
Abstract

The Veterinary Laboratory Europe (VLE) deployed a direct, rapid immunohistochemical test (DRIT), developed by the Centers for Disease Control and Prevention (CDC), to the US military veterinary units in Iraq and Afghanistan. The test detects rabies virus antigen in fresh and frozen brainstem samples from various animal species. The goal was to conduct surveillance in 2 countries without current rabies diagnostic capabilities and little historical data on rabies prevalence. During the deployment the DRIT was evaluated, and Veterinary Corps officers and technicians were trained to operate the test. Civilian veterinarians from both Iraq and Afghanistan were organized for a lecture forum on rabies, a sample collection lab, and familiarization with the DRIT. Samples collected in Iraq and Afghanistan were tested in the field with the DRIT, and compared to the traditional laboratory standard utilizing direct fluorescent antibody testing at VLE and the CDC with 100% agreement.

摘要

欧洲兽医实验室(VLE)向驻伊拉克和阿富汗的美军兽医部队部署了由美国疾病控制与预防中心(CDC)研发的直接快速免疫组化检测(DRIT)。该检测可在来自各种动物物种的新鲜和冷冻脑干样本中检测狂犬病病毒抗原。目标是在两个目前没有狂犬病诊断能力且狂犬病流行历史数据很少的国家开展监测。在部署期间对DRIT进行了评估,并对兽医部队的军官和技术人员进行了操作该检测的培训。来自伊拉克和阿富汗的文职兽医被组织参加了一个关于狂犬病的讲座论坛、一个样本采集实验室,并熟悉了DRIT。在伊拉克和阿富汗采集的样本在现场用DRIT进行检测,并与在VLE和CDC使用直接荧光抗体检测的传统实验室标准进行比较,结果完全一致。

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