Ngaingan 病毒，一种与袋鼠相关的弹状病毒，包含第二个糖蛋白基因和七个新的开放阅读框。

Ngaingan virus, a macropod-associated rhabdovirus, contains a second glycoprotein gene and seven novel open reading frames.

机构信息

CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Victoria, Australia; School of Chemistry and Molecular Sciences, University of Queensland, St. Lucia, Queensland, Australia; Human Protection and Performance Division, Defence Science and Technology Organisation, Melbourne, Australia; Australian Biosecurity Cooperative Research Centre for Emerging Infectious Disease, Brisbane, Queensland, Australia.

Northern Territory Department of Regional Development, Primary Industry, Fisheries and Resources, Berrimah Veterinary Laboratories, Berrimah, Northern Territory, Australia.

出版信息

Virology. 2010 Mar 30;399(1):98-108. doi: 10.1016/j.virol.2009.12.013. Epub 2010 Jan 20.

DOI:10.1016/j.virol.2009.12.013

PMID:20089287

Abstract

Ngaingan virus (NGAV) was isolated from a pool of biting midges that were collected in the tropics of northern Australia. Reported here is the full-length sequence of the NGAV genome, which, at over 15.7 kb, is the largest in any rhabdovirus described to date and contains 13 genes, the highest number of genes observed in any (-) ssRNA virus. Seven of these putative genes show no significant homology to known proteins. Like viruses in the genus Ephemerovirus, NGAV possesses a second glycoprotein gene (G(NS)). Phylogenetic analyses, however, place NGAV within the yet to be classified "Hart Park" group containing Wongabel and Flanders viruses, which do not contain a second glycoprotein gene. Screening of various animal sera from northern Australia has indicated that NGAV is currently circulating in macropods (wallabies, wallaroos and kangaroos), highlighting the need for further studies to determine its potential to cause disease in these species.

摘要

Ngaingan 病毒（NGAV）是从澳大利亚北部热带地区采集的吸血蠓中分离出来的。本文报道了 NGAV 基因组的全长序列，全长超过 15.7kb，是迄今为止描述的所有弹状病毒中最大的，包含 13 个基因，是所有（-）ssRNA 病毒中观察到的基因数量最多的。其中 7 个推定基因与已知蛋白没有显著同源性。与属内的 Ephemerovirus 病毒一样，NGAV 拥有第二个糖蛋白基因（G(NS)）。然而，系统发育分析将 NGAV 置于尚未分类的“Hart Park”组内，该组包含 Wongabel 和 Flanders 病毒，它们不包含第二个糖蛋白基因。对来自澳大利亚北部的各种动物血清的筛选表明，NGAV 目前在有袋类动物（沙袋鼠、小袋鼠和袋鼠）中传播，这突出表明需要进一步研究来确定其在这些物种中引起疾病的潜力。